Phospho Antibodies | St John's Laboratory

Anti-Phospho-p53-Ser15 antibody [PT0901R] (STJA0039935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p53-Ser392 antibody [PT1223R] (STJA0040171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p53-Ser46 antibody [PT0903R] (STJA0039796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-p53-Ser6 antibody [PT1249R] (STJA0040157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-p53-T18 antibody [23#] (STJA0019168)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of lysates from C6 cells, using Phospho-p53-T81 Rabbit pAb (STJ11103770) at 1:500 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-p53-T81 antibody (STJ11103770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot of Jurkat cell lysate showing specific immunolabeling of the ~48 kDa p62 phosphorylated at Ser28 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-p62-Ser28 antibody (STJA0003736)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£454.50

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Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-p62-Thr269/Ser272 antibody (STJA0003737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£454.50

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Anti-Phospho-p70 S6 Kinase 1-S424 antibody (STJ11107635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-p70 S6 Kinase 1-T421 antibody (STJ11107636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-p70 S6 Kinase 1-T421/S424 antibody (STJ11107646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-p70 S6 kinase Beta-Ser371 antibody [PT0919R] (STJA0039808)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p70 S6 Kinase-Thr389 antibody [PT0721R] (STJA0039840)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p90RSK-Ser380 antibody [PT0759R] (STJA0039441)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p90RSK-Thr359/Ser363 antibody [PT0755R] (STJA0039487)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from HUVEC cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S352 Rabbit pAb (STJ11107616). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-p90Rsk/RSK1/RPS6KA1-S352 antibody (STJ11107616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-p90Rsk/RSK1/RPS6KA1-T573 antibody (STJ11107649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from mouse brain, using PAK1/2/3 (Phospho-Ser144/141/139) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK Alpha/Beta/Gamma-Ser144/141/139 antibody (111-160 aa) (STJ90827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK1/2/3 (Phospho-Thr423/402/421) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PAK Alpha/Beta/Gamma-Thr423/402/421 antibody (391-440 aa) (STJ91121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68 kDa to ~70 kDa PAK protein phosphorylated at Ser402 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-PAK1-3-Thr402 antibody (STJA0003739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Canine/Human

£454.50

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Anti-Phospho-PAK1-S141 antibody (STJ11107644)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-PAK1-S199/S204 + S192/S197 antibody (STJ11107648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-PAK1-S199/S204 antibody (STJ11101158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAK1 (Phospho-Ser199) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK1-Ser199 antibody (165-214 aa) (STJ90917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-PAK1-Ser199/204 antibody (STJ196376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-PAK1-Ser20 antibody (STJ196377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 3T3 cells using Phospho-PAK Alpha (S204) Polyclonal Antibody

Anti-Phospho-PAK1-Ser204 antibody (170-219 aa) (STJ90954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of Raji cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PAK1-Ser21 antibody (STJA0010056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-PAK1-T212 pAb (STJ114842) at 1:1000 dilution. 293T cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PAK1-T212 antibody (STJ114842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-PAK1-T402 antibody (STJ11107645)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 293 cells using Phospho-PAK Alpha (T212) Polyclonal Antibody

Anti-Phospho-PAK1-Thr212 antibody (178-227 aa) (STJ90826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68 kDa PAK1 protein phosphorylated at Thr84 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 minutes).

Anti-Phospho-PAK1-Thr84 antibody (STJA0003738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Guinea Pig/Non-Human Primates/Sheep/Zebrafish

£454.50

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Western blot analysis of various lysates using Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb (STJ11102649) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PAK1/PAK2/PAK3-S144/S141/S139 antibody [S9MR] (STJ11102649)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-PAK2-S20 Rabbit pAb (STJ117901) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PAK2-S20 antibody (STJ117901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of C6, using Phospho-PAK2-S20 Rabbit monoclonal antibody (STJ11105893) at 1:2000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-PAK2-S20 antibody [S5893RM] (STJ11105893)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 24h/Jurkat cells treated with PMA 125ng/ml 30', using PAK2 (Phospho-Ser141) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser141 antibody (107-156 aa) (STJ90590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using PAK2 (Phospho-Ser192) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser192 antibody (158-207 aa) (STJ90589)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK2 (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser197 antibody (163-212 aa) (STJ91214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-PAK Gamma (S20) Polyclonal Antibody

Anti-Phospho-PAK2-Ser20 antibody (5-54 aa) (STJ90860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from rat heart, using PAK3 (Phospho-Ser154) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK3-Ser154 antibody (121-170 aa) (STJ90955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PAK4-Ser474 antibody (441-490 aa) (STJ91133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (STJ11102619) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PAK4/PAK5/PAK6-S474/S560/S602 antibody [S9MR] (STJ11102619)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from LOVO cells treated with PMA 125ng/ml 30', using PAK5/6 (Phospho-Ser602/Ser560) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK5/6-Ser602/S560 antibody (566-615 aa) (STJ90635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-PAK6-Ser-165 antibody (STJA0005511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£367.50

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Western blot of mouse whole brain lysate showing specific labeling of the ~86 kDa PanK4 phosphorylated at Ser63 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is nearly eliminated with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-PANK4-Thr63 antibody (STJA0003740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Hamster/Rat

£454.50

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Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S101 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser101.

Anti-Phospho-PARK2-Ser101 antibody (STJA0003742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

£454.50

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Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S378 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser378.

Anti-Phospho-PARK2-Ser378 antibody (STJA0003743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

£454.50

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Western Blot analysis of 1 Jurkat cell 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PASK-Thr1165 antibody (STJA0009956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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