• Western blot analysis of lysates from Jurkat cells treated with Ca+ 40nM 30', using MYOD (Phospho-Ser200) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MYOD1-Ser200 antibody (171-220 aa) (STJ90472)

SKU:
STJ90472

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Myoblast determination protein 1-Ser200 (171-220 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYOD1
Gene ID: 4654
Uniprot ID: MYOD1_HUMAN
Immunogen Region: 171-220 aa
Specificity: Phospho-MyoD (S200) Polyclonal Antibody detects endogenous levels of MyoD protein only when phosphorylated at S200.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MYOD around the phosphorylation site of Ser200 at the amino acid range 171-220
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Protein Name Myoblast Determination Protein 1
Class C Basic Helix-Loop-Helix Protein 1
Bhlhc1
Myogenic Factor 3
Myf-3
Database Links Reactome: R-HSA-525793
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Myoblast Determination Protein 1 antibody
Anti-Class C Basic Helix-Loop-Helix Protein 1 antibody
Anti-Bhlhc1 antibody
Anti-Myogenic Factor 3 antibody
Anti-Myf-3 antibody
Anti-MYOD1 antibody
Anti-BHLHC1 antibody
Anti-MYF3 antibody
Anti-MYOD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance