• Western blot analysis of lysates from HeLa cells treated with nocodazole 1ug/ml 18h, using p47 phox (Phospho-Ser359) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of HeLa nocodazole 1ug/ml 18h cells using Phospho-p47-phox (S359) Polyclonal Antibody diluted at 1：500

Anti-Phospho-NCF1-Ser359 antibody (331-380 aa) (STJ91104)

SKU:
STJ91104

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Neutrophil cytosol factor 1-Ser359 (331-380 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCF1
Gene ID: 653361
Uniprot ID: NCF1_HUMAN
Immunogen Region: 331-380 aa
Specificity: Phospho-p47-phox (S359) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S359.
Immunogen: The antiserum was produced against synthesized peptide derived from the human p47 phox around the phosphorylation site of Ser359 at the amino acid range 331-380
Post Translational Modifications Phosphorylated by PRKCD.phosphorylation induces activation of NCF1 and NADPH oxidase activity.
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Protein Name Neutrophil Cytosol Factor 1
Ncf-1
47 Kda Autosomal Chronic Granulomatous Disease Protein
47 Kda Neutrophil Oxidase Factor
Ncf-47k
Neutrophil Nadph Oxidase Factor 1
Nox Organizer 2
Nox-Organizing Protein 2
Sh3 And Px Domain-Containing Protein 1a
P47-Phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Neutrophil Cytosol Factor 1 antibody
Anti-Ncf-1 antibody
Anti-47 Kda Autosomal Chronic Granulomatous Disease Protein antibody
Anti-47 Kda Neutrophil Oxidase Factor antibody
Anti-Ncf-47k antibody
Anti-Neutrophil Nadph Oxidase Factor 1 antibody
Anti-Nox Organizer 2 antibody
Anti-Nox-Organizing Protein 2 antibody
Anti-Sh3 And Px Domain-Containing Protein 1a antibody
Anti-P47-Phox antibody
Anti-NCF1 antibody
Anti-NOXO2 antibody
Anti-SH3PXD1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance