Post Translational Modifications | Primary antibodies

Immunofluorescence analysis of HeLa cells using MonoMethyl-NONO/p54nrb-R357/R365 pAb (STJ110387) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-NONO-R357/R365 antibody (STJ110387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HT29 cells using Phospho-JAK2-Y1007 Rabbit pAb (STJ110832). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-JAK2-Y1007 antibody (STJ110832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-LIMK1-T508 Rabbit pAb (STJ110833).

Anti-Phospho-LIMK1-T508 antibody (STJ110833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (STJ110835) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody (STJ110835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HL-60 and HL-60 cells, using Phospho-ALK-Y1096 Rabbit pAb (STJ110843) at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight. HL-60 cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ALK-Y1096 antibody (STJ110843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-IKK Alpha-S180 Rabbit pAb (STJ110846) at 1:1000 dilution. HeLa cells were treated by both TNF-Alpha (20ng/mL) and Calyculin A (100nM) for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CHUK-S180 antibody (STJ110846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from A-431 cells, using Phospho-Fyn-S21 Rabbit pAb (STJ110850) at 1:1000 dilution. A-431 cells were treated by 10% FBS after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-FYN-S21 antibody (STJ110850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-GSK3 Beta-Y216 + GSK3 Alpha-Y279 Rabbit pAb (STJ110852) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-GSK3B-Y279 antibody (STJ110852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-eNOS-S1177 Rabbit pAb (STJ110855) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-NOS3-S1177 antibody (STJ110855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of various lysates, using Phospho-AURKA-T288 Rabbit pAb (STJ110859) at 1:1000 dilution. HeLa were treated by Hydroxyurea (4mM) or treated by Nocodazole (50ng/mL) for 20 hours. HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-AURKA-T288 antibody (STJ110859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-ATF2-T69/T71 Rabbit pAb (STJ110861) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit

Anti-Phospho-ATF2-T69/T71 antibody (STJ110861)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (STJ110862) at 1:18000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-MAPK14-T180/Y182 antibody (STJ110862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HER2/ErbB2-Y1221/1222 Rabbit pAb (STJ110863) at 1:1000 dilution. HeLa cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-ERBB2-Y1221/1222 antibody (STJ110863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from A-431 cells, using Phospho-FLT3-Y589/591 Rabbit pAb (STJ110865) at 1:500 dilution. A-431 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-FLT3-Y589/591 antibody (STJ110865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from RAW264. 7 cells, using Phospho-Jak1-Y1034/Y1035 Rabbit pAb (STJ110866) at 1:1000 dilution. RAW264. 7 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-JAK1-Y1022/1023 antibody (STJ110866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MAP2K4-S257/T261 Rabbit pAb (STJ110871) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K4-S257/T261 antibody (STJ110871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-IRS1-S307 (Human Specific) Rabbit pAb (STJ110879) at 1:1000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-IRS1-S307 antibody (STJ110879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K23 antibody (STJ11100124) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Acetyl-Histone H3-K23 antibody (STJ11100124)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Dot-blot analysis of all sorts of methylation peptides using pan Symmetric-DiMethyl-Arginine antibody (STJ11100217) at 1:1000 dilution.

Anti-Pan-Di-Methyl-Rme2s antibody (STJ11100217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using pan-TriMethyl-lysine polyclonal antibody (STJ11100248) at 1:500 dilution. HeLa and MCF7 cells were treated by ADOX (100 Mu M) for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Pan-Tri-Methyl-Kme3 antibody (STJ11100248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine antibody (STJ11100249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Pan-Mono-Methyl-Kme1 antibody (STJ11100249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit polyclonal antibody (STJ11100252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Pan-Di-Methyl-Kme2 antibody (STJ11100252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using pan-Mono-MethylR (R

Anti-Pan-Mono-Methyl-Rme1 antibody (STJ11100253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using MonoMethyl-YAP1-K342 Rabbit polyclonal antibody (STJ11100581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Mono-Methyl-YAP1-K342 antibody (STJ11100581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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The m6A mouse monoclonal antibody (STJ11100594) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine (100ng for each oligomer). Oligomer 8- N6-methyladenosine (ATAACTGG-m6A-CCGAATGG) Oligomer 9- unmodified adenosine (ATAACTGGACCGAATGG) Oligomer 10- unmodified adenosine (AAAAAAAAAAAAAAAA-biotin)

Anti-N6-Methyl-m6A antibody [S4MM] (STJ11100594)

Clonality :

Monoclonal

Host :

Mouse

Applications :

DB/ELISA

Reactivity :

Species Independent

£118.50 - £521.50

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Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ11100898). Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-R3 antibody (STJ11100898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K64 Rabbit polyclonal antibody (STJ11100934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H3-K64 antibody (STJ11100934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K91 Rabbit polyclonal antibody (STJ11100937) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H4-K91 antibody (STJ11100937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-MAP3K5-S83 Rabbit pAb (STJ11100961) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour, treated by TNF-Alpha (20ng/mL) for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP3K5-S83 antibody (STJ11100961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from NIH3T3 cells, using Phospho-TrkA-Y490 Rabbit pAb (STJ11100963). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NTRK1-Y490 antibody (STJ11100963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDGFRB-Y751 Rabbit pAb (STJ11100964) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PDGFRB-Y751 antibody (STJ11100964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-Beta-Catenin-S33/S37/T41 Rabbit pAb (STJ11100967) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-CTNNB1-S33/S37/T41 antibody (STJ11100967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MET-Y1234/1235 Rabbit pAb (STJ11100968) at 1:1000 dilution. HeLa cells were treated by HGF (40 ng/ml) at 37 °C for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MET-Y1234/1235 antibody (STJ11100968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Chk2-S516 Rabbit pAb (STJ114888). NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CHEK2-S516 antibody (STJ11100971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-PPP1R12A-S668 Rabbit pAb (STJ11100976) at 1:2000 dilution. Both HeLa cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PPP1R12A-S668 antibody (STJ11100976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates, using Phospho-NPM1-T199 Rabbit pAb (STJ11100977) at 1:900 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-NPM1-T199 antibody (STJ11100977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-PLC beta 3 (PLCB3) -S1105 Rabbit pAb (STJ11100978) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PLCB3-S1105 antibody (STJ11100978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-kB p65/RelA-T434 pAb (STJ11100979) at 1:1000 dilution or RelA antibody (STJ1177811). NIH/3T3 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5min.

Anti-Phospho-RELA-T434 antibody (STJ11100979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb (STJ11100980) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S28 antibody (STJ11100980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-Histone H3-S10 Rabbit pAb (STJ11100981) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S10 antibody (STJ11100981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-PPIG-S376 Rabbit pAb (STJ11100983) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PPIG-S376 antibody (STJ11100983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (STJ11100984) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-NEDD4L-S448 antibody (STJ11100984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-SF3B1-T313 Rabbit pAb (STJ11100985) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-SF3B1-T313 antibody (STJ11100985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-EEF2K-S366 Rabbit pAb (STJ11100986) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-Phospho-EEF2K-S366 antibody (STJ11100986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-Histone H3-T3 pAb (STJ11100987) at 1:1000 dilution. C6 and HeLa cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. C6 and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-T3 antibody (STJ11100987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-TBK1/NAK-S172 Rabbit pAb (STJ11100988) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-TBK1-S172 antibody (STJ11100988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-DRP1-S616 Rabbit pAb (STJ11100990) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-DNM1L-S616 antibody (STJ11100990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from Mouse brown fat using Phospho-HSL-S563 Rabbit pAb (STJ11100992) at 1:5000 dilution incubated overnight at 4 °C. Mouse brown fat were treated by Lambda-PP mixed solution (1 Mu l) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-LIPE-S563 antibody (STJ11100992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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