• Western blot analysis of extracts of various cell lines, using Phospho-PPIG-S376 antibody (STJ11100983) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using Phospho-PPIG-S376 Rabbit polyclonal antibody (STJ11100983) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-PPIG-S376 antibody (STJ11100983)

SKU:
STJ11100983

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PPIG-S376 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPIG
Gene ID: 9360
Uniprot ID: PPIG_HUMAN
Immunogen: A synthetic phosphorylated peptide around S376 of human PPIG (NP_004783.2).
Immunogen Sequence: VSSGE
Tissue Specificity Ubiquitous.
Function PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase G
Ppiase G
Peptidyl-Prolyl Isomerase G
Casp10
Clk-Associating Rs-Cyclophilin
Cars-Cyp
Cars-Cyclophilin
Sr-Cyclophilin
Sr-Cyp
Srcyp
Cyclophilin G
Rotamase G
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-9692916
Cellular Localisation Nucleus Matrix
Nucleus Speckle
Colocalizes With Rna Splicing Factors At Nuclear Speckles
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase G antibody
Anti-Ppiase G antibody
Anti-Peptidyl-Prolyl Isomerase G antibody
Anti-Casp10 antibody
Anti-Clk-Associating Rs-Cyclophilin antibody
Anti-Cars-Cyp antibody
Anti-Cars-Cyclophilin antibody
Anti-Sr-Cyclophilin antibody
Anti-Sr-Cyp antibody
Anti-Srcyp antibody
Anti-Cyclophilin G antibody
Anti-Rotamase G antibody
Anti-PPIG antibody

Information sourced from Uniprot.org

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