• Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody (STJ11100986) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 15s.
  • Western blot analysis of extracts of various cell lines, using Phospho-EEF2K-S366 antibody (STJ11100986) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 polyclonal antibody (STJ11100986) at 1:1000 dilution or EEF2K antibody (STJ27357). Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-Phospho-EEF2K-S366 antibody (STJ11100986)

SKU:
STJ11100986

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-EEF2K-S366 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF2K
Gene ID: 29904
Uniprot ID: EF2K_HUMAN
Immunogen: A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1).
Immunogen Sequence: TLSGS
Post Translational Modifications Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.
Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Protein Name Eukaryotic Elongation Factor 2 Kinase
Eef-2 Kinase
Eef-2k
Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase
Database Links Reactome: R-HSA-166208
Alternative Antibody Names Anti-Eukaryotic Elongation Factor 2 Kinase antibody
Anti-Eef-2 Kinase antibody
Anti-Eef-2k antibody
Anti-Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase antibody
Anti-EEF2K antibody

Information sourced from Uniprot.org

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