Rabbit Monoclonal Antibodies | St John's Laboratory

Western blot analysis of NIH/3T3, using Phospho-EIF4EBP1-S65 antibody (STJ11105846) at 1:20000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-S65 antibody [S5846RM] (STJ11105846)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts from C6 cells, using Phospho-eIF4EBP1-T37 Rabbit monoclonal antibody (STJ11105858) at 1:1000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-EIF4EBP1-T37 antibody [S5858RM] (STJ11105858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£118.50 - £521.50

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Western blot analysis of extracts from C6 cells, using Phospho-eIF4EBP1-T37/T46 Rabbit monoclonal antibody (STJ11105857) at 1:7000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-T37/T46 antibody [S5857RM] (STJ11105857)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£118.50 - £521.50

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Western blot analysis of extracts of NIH/3T3, using Phospho-eIF4EBP1-T70 antibody (STJ11105824) at1:5000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EIF4EBP1-T70 antibody [S5824RM] (STJ11105824)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Phospho-Elongation Factor 2-Thr56/Thr58 antibody [R02-5B5] (STJA0035061)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-eNOS-Ser1177 antibody [PT0906R] (STJA0039917)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-EPHA2-S897 antibody [SRM13704] (STJ11108434)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-ErbB 2-Tyr1221/Tyr1222 antibody [R87-5Y-7] (STJA0035700)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-ErbB 2/EGFR-Tyr1248/Tyr1173 antibody [R03-6A8] (STJA0033910)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-ErbB 4-Tyr1162 antibody [R07-4C0] (STJA0035064)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-ErbB-3-Tyr1289 antibody [PT0990R] (STJA0039843)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-Phospho-ErbB2-Tyr1139 antibody [R78-8J-4] (STJA0035681)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from A-431 cells, using Phospho-HER2/ErbB2-Y1139 (STJ11103785) at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ErbB2-Y1139 antibody [S5MR] (STJ11103785)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of A-431, using Phospho-ErbB2 (Y1221 + Y1222) Rabbit monoclonal antibody (STJ11105879) at 1:1000 dilution. A-431 cells were treated by EGF (200 ng/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-ERBB2-Y1221/Y1222 antibody [S5879RM] (STJ11105879)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Anti-Phospho-ErbB3-Tyr1222 antibody [R53-7S-8] (STJA0035676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (STJ11102622) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ERBB3-Y1222 antibody [S2MR] (STJ11102622)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of extracts from MCF7 cells, using Phospho-ErbB3/HER3-Y1289 Rabbit monoclonal antibody (STJ11105873) at 1:1000 dilution. MCF7 cells were treated by neuregulin at 37 °C for 15 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-Phospho-ERBB3-Y1289 antibody [S5873RM] (STJ11105873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospho-ErbB4/HER4-Y1284 antibody [SRM13706] (STJ11108436)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Anti-Phospho-Erk 1/2-Thr202/Tyr204 antibody [PT0643R] (STJA0039299)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R05-2H9] (STJA0033905)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Hamster/Rat

£133.50 - £281.50

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Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R07-3G4] (STJA0033913)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R49-1Y-4] (STJA0035674)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-ERK1/2-Thr202/Tyr204+Thr185/Tyr187 antibody [R08-8S3] (STJA0035749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-ERK1/2/-Thr202/Tyr204+Thr185/Tyr187 antibody [R04-9E9] (STJA0034288)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (STJ11102556) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-ERK1/ERK2-T202/T185 antibody [S6MR] (STJ11102556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ESR Alpha-S118 Rabbit mAb (STJ11102593) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ESR1-S118 antibody [S3MR] (STJ11102593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£143.50 - £618.50

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Anti-Phospho-Estrogen Receptor alpha-Ser118 antibody [R03-3T5] (STJA0036000)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-Ezrin/Radixin/Moesin-Thr567/Thr564/Thr558 antibody [PT0916R] (STJA0040055)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-Ezrin/Radixin/Moesin-Thr567/Thr564/Thr558 antibody [R03-1G3] (STJA0035065)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat/Hamster

£133.50 - £281.50

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Anti-Phospho-FADD-S194 antibody (STJA0033686)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£224.50 - £319.50

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Anti-Phospho-FAK-Tyr397 antibody [PT0915R] (STJA0039806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-FAK-Tyr397 antibody [R01-3L2] (STJA0035977)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-FAK-Tyr397 antibody [R05-1A1] (STJA0033930)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Rat

£133.50 - £281.50

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Anti-Phospho-FAK-Tyr576 antibody [R08-3A7] (STJA0035066)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-FAK-Tyr576/Tyr577 antibody [PT1269R] (STJA0040090)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-FAK-Y576/Y577 antibody [SRM13711] (STJ11108441)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£0.00

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Anti-Phospho-FGFR3-Tyr724 antibody [R68-1C-2] (STJA0035680)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-Filamin A/B-Ser2152/Ser2107 antibody [R02-7C0] (STJA0035067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-Phospho-Flk-1/VEGFR2-Tyr1175 antibody [PT0865R] (STJA0039919)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£285.00 - £540.00

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Anti-Phospho-FOXO1 + FOXO3A+ FOXO4-T24+T32+T28 antibody [SRM13687] (STJ11108417)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£0.00

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Western blot analysis of Phospho-FOXO3A (Ser253) in 3T3 lysates using Phospho-FOXO3A (Ser253) antibody.

Anti-Phospho-FOXO3-Ser253 antibody (248-260 aa) [R06-2G6] (STJA0011330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-FOXO3A-Ser253 antibody [PT0061R] (STJA0039645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-FOXO3A-Ser253 antibody [R08-7U1] (STJA0035964)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-GATA3-S308-S308 antibody [SRM15115] (STJ11109845)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£0.00

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Anti-Phospho-GATA3-Ser308 antibody [R05-3G0] (STJA0035068)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-GCN2-Thr899 antibody [R08-4N7] (STJA0035767)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-Glucocorticoid Receptor-Ser226 antibody [R02-1H0] (STJA0035069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-Phospho-Glutamate Receptor 1-Ser845 antibody [R03-7G4] (STJA0034014)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£133.50 - £281.50

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