Loading Control Antibody | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded rat testis using PCNA Rabbit polyclonal antibody (STJ115299) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PCNA antibody (162-261) (STJ115299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded rat testis using PCNA Rabbit polyclonal antibody (STJ24918) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PCNA antibody (162-261) (STJ24918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-PCNA antibody (162-261) [S3MR] (STJ11101413)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/African green monkey

$198.03 - $853.53

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Western blot analysis of lysates from 1) HepG2, 2) HuvEC, 3) COS7 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-PCNA antibody (61-110 aa) (STJ94982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Rabbit

$67.62 - $360.18

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Anti-PCNA antibody (C-Term) (STJ13101593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$457.47

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Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of follicular center cells

Anti-PCNA antibody (Full length) [ZR378] (STJ180587)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$222.87 - $736.23

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STJ72848 (0. 01µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PCNA antibody (Internal) (STJ72848)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$431.25

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Western blot analysis of Hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour) cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PCNA antibody (Internal) (STJ98857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-PCNA antibody [12D10] {AbFluor™ 350} (STJA0006137)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {AbFluor™ 405} (STJA0006138)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {AbFluor™ 488} (STJA0006139)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {AbFluor™ 555} (STJA0006140)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {AbFluor™ 594} (STJA0006141)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {AbFluor™ 647} (STJA0006142)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {AbFluor™ 680} (STJA0006143)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {Biotin} (STJA0006144)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {Cy3} (STJA0006145)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {Cy5} (STJA0006146)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {FITC} (STJA0006147)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [12D10] {HRP} (STJA0006148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

$205.62 - $342.24

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Anti-PCNA antibody [1A041] (STJA0019917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

$229.77 - $313.95

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Western blot detection of PCNA in Hela, 3T3, COS7, C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

Anti-PCNA antibody [2E1-G10-H10] (STJ99203)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC/IHC

Reactivity :

Human/Mouse/Monkey/Rat/Hamster

$205.62 - $496.80

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Anti-PCNA antibody [2G2] (STJ16103540)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

$1,030.17

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Anti-PCNA antibody [9C6] {HRP} (STJA0042572)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$178.71 - $278.07

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Anti-PCNA antibody [IHC711] (STJA0042240)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

$215.97 - $617.55

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Anti-PCNA antibody [PC10] (STJ16103384)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-P/IHC-F/ICC

Reactivity :

Mouse/Human/Non-Human Primates/Drosophila/Chicken/Rat

$578.91

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Anti-PCNA antibody [PT0224R] (STJA0039630)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Western blot analysis of PCNA. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-PCNA antibody [SM4907] (STJA0004907)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ICC

Reactivity :

Human/Mouse/Rat

$235.29 - $322.23

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Anti-PCNA antibody [SQab18115] (STJ16102921)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IP/WB

Reactivity :

Other

$977.73

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Biotinylated STJ72849 (0. 05ug/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-PCNA, Biotinylated antibody (111-122) {Biotin} (STJ73513)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$431.25

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Biotinylated STJ72848 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-PCNA, Biotinylated antibody (Internal) {Biotin} (STJ73207)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$431.25

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Anti-Phospho-ACTB-Tyr-53 antibody (STJA0005242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$504.39

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Western blot analysis of various cells using Phospho-Calnexin (S583) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CANX-Ser583 antibody (543-592 aa) (STJ90201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using Phospho-Histone H3-S10 Rabbit pAb (STJ11100981) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S10 antibody (STJ11100981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.57 - $853.53

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Anti-Phospho-Histone H3-S10 antibody [SRM13680] (STJ11108410)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$0.00

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Western blot analysis of various lysates using Phospho-Histone H3-S10/T11 Rabbit pAb (STJ11101033) at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour and treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S10/T11 antibody (STJ11101033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.57 - $853.53

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Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb (STJ11100980) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S28 antibody (STJ11100980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.57 - $853.53

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Anti-Phospho-Histone H3-S28 antibody [SRM13693] (STJ11108423)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat/Other

$0.00

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Immunofluorescence analysis of Hela cell. 1, Histone H3 (phospho Ser10) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-Histone H3-Ser10 antibody (1-50 aa) (STJ90289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IHC-F/FC/ICC/IF/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-Histone H3-Ser28 antibody (13-62 aa) (STJ90290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from C6 cells using Phospho-Histone H3-T11 Rabbit pAb (STJ22169) at 1:2000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Histone H3-T11 antibody (STJ22169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.57 - $853.53

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Anti-Phospho-Histone H3-T11 antibody [N123/19] (STJA0019175)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$289.11 - $1,840.23

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Anti-Phospho-Histone H3-T11 antibody [SRM13698] (STJ11108428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ChIP/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western blot analysis of various lysates using Phospho-Histone H3-T3 pAb (STJ11100987) at 1:1000 dilution. C6 and HeLa cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. C6 and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-T3 antibody (STJ11100987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3. 3-T3 Rabbit mAb (STJ11102645) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-Histone H3-T3 antibody [S5MR] (STJ11102645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

$163.53 - $719.67

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Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-Histone H3-T32 Rabbit pAb (STJ11103719) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-Histone H3-T32 antibody (STJ11103719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.57 - $853.53

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Western blot analysis of various lysates using Phospho-Histone H3-T45 Rabbit pAb (STJ11101034) at 1:1000 dilution. Hela cells, NIH/3T3 cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Histone H3-T45 antibody (STJ11101034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.57 - $853.53

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Western blot analysis of lysates from C2C12 cells, using Phospho-Histone H3-T6 Rabbit pAb (STJ11101035) at 1:400 dilution. C2C12 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-Histone H3-T6 antibody (STJ11101035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.57 - $853.53

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Loading Control Antibodies