| Host: | Mouse |
| Applications: | WB/IF/IHC |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Proliferating cell nuclear antigen is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | 12D10 |
| Conjugation: | HRP |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS pH7.4, containing 0.02% Sodium Azide and 50% Glycerol. |
| Purification: | The antibody was purified using affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | Optimal working dilutions should be determined experimentally by the investigator Suggested starting dilutions are as follows: WB 1:5000 IHC 1:200 |
| Storage Instruction: | Stable for one year at-15°C to-25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezi |
| Gene Symbol: | PCNA |
| Gene ID: | 5111 |
| Uniprot ID: | PCNA_HUMAN |
| Specificity: | PCNA Monoclonal Antibody (12D10) HRP Conjugated, specially designed for your Western blot analysis. |
| Function | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Protein Name | Proliferating Cell Nuclear AntigenPcnaCyclin |
| Database Links | Reactome: R-HSA-110312Reactome: R-HSA-110314Reactome: R-HSA-110320Reactome: R-HSA-1362277Reactome: R-HSA-174411Reactome: R-HSA-174414Reactome: R-HSA-174417Reactome: R-HSA-174437Reactome: R-HSA-4615885Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5651801Reactome: R-HSA-5655862Reactome: R-HSA-5656121Reactome: R-HSA-5656169Reactome: R-HSA-5685942Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6804114Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183Reactome: R-HSA-69205Reactome: R-HSA-8866654 |
| Cellular Localisation | NucleusColocalizes With CrebbpEp300 And Pold1 To Sites Of Dna DamageForms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S PhaseCo-Localizes With Smarca5/Snf2h And Baz1b/Wstf At Replication Foci During S PhaseTogether With Apex2Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents |
| Alternative Antibody Names | Anti-Proliferating Cell Nuclear Antigen antibodyAnti-Pcna antibodyAnti-Cyclin antibodyAnti-PCNA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
