Western Blot Antibodies | St John's Laboratory

Western blot analysis of Beclin 1 in K562, rat Brain, C6, 3T3, Hela lysates using Beclin 1 antibody.

Anti-BECN1 antibody (1-200 aa) [R02-8D5] (STJA0010822)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

€308.13

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Immunofluorescence analysis of PC-12 cells using [KO Validated] Beclin 1 Rabbit polyclonal antibody (STJ11104484) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BECN1 antibody (1-280) (STJ11104484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of extracts of various cell lines, using Beclin 1 rabbit polyclonal antibody (STJ113348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BECN1 antibody (1-280) (STJ113348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of various lysates, using [KO Validated] Beclin 1 Rabbit pAb (STJ29492) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-BECN1 antibody (1-450) (STJ29492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from 1) mouse-brain, 2) mouse-lung, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BECN1 antibody (110-190 aa) (STJ98594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Chicken

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.

Anti-BECN1 antibody (110-190 aa) [5C2] (STJ97761)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse/Cow

€60.00 - €315.00

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Western blot analysis of various lysates using Beclin 1 Rabbit polyclonal antibody (STJ11101202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BECN1 antibody (25-450) (STJ11101202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis using Beclin-1 monoclonal antibody against Mouse brain, A431, HeLa cell lysate.

Anti-BECN1 antibody (STJ98472)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Pig/Rabbit

€180.00 - €435.00

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Western blot analysis using Beclin-1 monoclonal antibody against HeLa (1) , A431 (2) , MCF-7 (3) , RAJI (4) , Jurkat (5) and SKBR-3 (6) cell lysate.

Anti-BECN1 antibody [2A4] (STJ97862)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

€180.00 - €435.00

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Western blot detection of Beclin 1 in Hela, 3T3, C6, COS7, CHO-K1, Rat brain and Jurkat cell lysates using Beclin 1 mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:55KDa.

Anti-BECN1 antibody [4F4-B10-C9] (STJ99148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Monkey/Hamster

€180.00 - €435.00

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Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser295. This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A: Mut) expressed in HeLa cell lysate.

Anti-BECN1-Ser295 antibody (STJA0003836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Xenopus/Zebrafish

€568.13

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BECN2 antibody (STJ192027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of HeLa cells, using BEGIN Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BEGAIN antibody (511-560 aa) (STJ91849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BEND2 antibody (48-98 aa) (STJ195723)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-BEND3 antibody (596-828 aa) (STJ11107503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-BEND3 antibody (STJA0008545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using CCDC4 Polyclonal Antibody

Anti-BEND4 antibody (371-420 aa) (STJ92068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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STJ73012 (0. 3µg/ml) staining of Daudi (A) , Human Tonsil (B) , Human Spleen (C) and Mouse Spleen (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BEND5 antibody (C-Term) (STJ73012)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Anti-BEND5 antibody (STJA0008941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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STJ70324 (0. 3µg/ml) staining of Human Cerebellum (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-BERP/RNF22 antibody (N-Term) (STJ70324)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Anti-BES1 antibody (89 aa) (STJ11106195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana/Rice

€183.13 - €803.13

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Western blot analysis of 1) PC3, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with Bestrophin-1 Rabbit pAb diluted at 1:2,000.

Anti-BEST1 antibody (161-211 aa) (STJ197429)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using BEST1 antibody (STJ111168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BEST1 antibody (171-270) (STJ111168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using BEST1 antibody (STJ116005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-BEST1 antibody (376-585) (STJ116005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using BEST1 antibody (STJ28302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-BEST1 antibody (376-585) (STJ28302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunohistochemistry of mouse tissue with Anti-BEST1 primary antibody (STJ500233) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BEST1 antibody (500-585) (STJ500233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human

€583.13

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Anti-BEST1 antibody (500-585) {Biotin} (STJ500243)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human

€660.63

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Anti-BEST1 antibody (500-585) {FITC} (STJ500244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human

€660.63

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Immunohistochemistry of mouse tissue with Anti-BEST1 primary antibody (STJ500232) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BEST1 antibody (Mid-region) (STJ500232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

€583.13

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Anti-BEST1 antibody (Mid-region) {Biotin} (STJ500241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

€660.63

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Anti-BEST1 antibody (Mid-region) {FITC} (STJ500242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

€660.63

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Immunohistochemistry of mouse tissue with Anti-BEST1 primary antibody (STJ500231) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BEST1 antibody (N-Term) (STJ500231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€583.13

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Anti-BEST1 antibody (N-Term) {Biotin} (STJ500239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Cell labelling with Anti-BEST1 Antibody (FITC) (STJ500240) and basolateral membranes, at 1:100 dilution in buffer.

Anti-BEST1 antibody (N-Term) {FITC} (STJ500240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Western blot with Anti-Bestrophin 2 Antibody (STJ500234) with peptide on at 1:500 dilution in buffer.

Anti-BEST2 antibody (388-404) (STJ500234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€534.38

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Anti-BEST2 antibody (388-404) {Biotin} (STJ500245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Anti-BEST2 antibody (388-404) {FITC} (STJ500246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain Tissue using BEST2 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-BEST2 antibody (STJ197430)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

€60.00 - €315.00

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Anti-BEST3 antibody (79-129) (STJA0006689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunohistochemistry with Anti-Bestrophin 3 Antibody (STJ500235) of Mouse at 1:100 dilution in buffer. PFA-Fixed, paraffin embedded. Incubation for 36 h at 4°C. Requires antigen retrieval for paraformaldehyde-fixed specimens.

Anti-BEST3 antibody (C-Term) (STJ500235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

€583.13

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Anti-BEST3 antibody (C-Term) (STJ500236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Monkey

€583.13

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Anti-BEST3 antibody (C-Term) {Biotin} (STJ500247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

€660.63

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Anti-BEST3 antibody (C-Term) {Biotin} (STJ500249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Monkey

€660.63

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Anti-BEST3 antibody (C-Term) {FITC} (STJ500248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

€660.63

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Anti-BEST3 antibody (C-Term) {FITC} (STJ500250)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Monkey

€660.63

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Western blot with Anti-BEST4 Antibody (STJ500237) with peptide on at dilution in buffer. Lane 1: Pre-immune serum, Lane 2: Affinity purified primary antibody

Anti-BEST4 antibody (400-500) (STJ500237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

€534.38

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Anti-BEST4 antibody (400-500) (STJ500238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human

€534.38

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Anti-BEST4 antibody (400-500) {Biotin} (STJ500251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

€660.63

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