Western Blot Antibodies | St John's Laboratory

Western blot analysis using ApoE monoclonal antibody against HEK293 (1) and ApoE (AA: 20-267) -hIgGFc transfected HEK293 (2) cell lysate.

Anti-APOE antibody [1H4] (STJ97839)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$194.40 - $469.80

Choose Options

Anti-APOF antibody (220-300) (STJ119159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Anti-APOF antibody (220-300) (STJ119160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$151.88 - $708.08

Choose Options

Western blot analysis of various cells using ApoF Polyclonal Antibody diluted at 1：1000

Anti-APOF antibody (221-270 aa) (STJ91641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

STJ72692 (0. 2µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOH antibody (145-157) (STJ72692)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

Choose Options

Western blot analysis of lysates from 22Rv1 cells, using APOH Rabbit polyclonal antibody (STJ22654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-APOH antibody (21-345) (STJ22654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

Choose Options

STJ73310 (1µg/ml) staining of Human Albumin-depleted Plasma lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOH antibody (Internal) (STJ73310)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog

$421.88

Choose Options

Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-APOH antibody (STJ192004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Western blot analysis of lysates from mouse liver, using APOL1 Rabbit pAb (STJ115338) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-APOL1 antibody (1-238) (STJ115338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

Choose Options

Western blot analysis of various cells using ApoL1 Polyclonal Antibody diluted at 1：1000

Anti-APOL1 antibody (261-310 aa) (STJ91642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

STJ72575 (0. 1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL1 antibody (297-309) (STJ72575)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

Choose Options

Confocal imaging of Hep G2 cells using APOL1 Rabbit monoclonal antibody (STJ11102075, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-APOL1 antibody (303-398) [S5MR] (STJ11102075)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.83 - $775.58

Choose Options

STJ71721 (0. 1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-APOL1 antibody (C-Term) (STJ71721)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

Choose Options

Western blot analysis using ApoL1 monoclonal antibody against human plasma (1).

Anti-APOL1 antibody [1D4] (STJ97840)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

Choose Options

Western blot analysis of various lysates using APOL2 Rabbit pAb (STJ114094) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-APOL2 antibody (170-270) (STJ114094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

Choose Options

Western blot analysis of various cells using ApoL2 Polyclonal Antibody

Anti-APOL2 antibody (191-240 aa) (STJ91643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

EB008916 (0. 03µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL2 antibody (C-Term) (STJ71722)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

Choose Options

Western blot analysis of various lysates using APOL3 Rabbit pAb (STJ115781) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-APOL3 antibody (1-331) (STJ115781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

Choose Options

STJ71723 (1µg/ml) staining of DAUDI lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL3 antibody (C-Term) (STJ71723)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

Choose Options

Immunofluorescence analysis of MCF7 cells, using APOL4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-APOL4 antibody (301-350 aa) (STJ91645)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

STJ71652 (0. 5µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

Anti-APOL4 antibody (C-Term) (STJ71652)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

Choose Options

STJ71724 (1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL5 antibody (C-Term) (STJ71724)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

Choose Options

Western blot analysis of extracts from K562 cells, using ApoL6 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-APOL6 antibody (240-320 aa) (STJ96424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

STJ71653 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL6 antibody (Internal) (STJ71653)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

Choose Options

STJ71725 (2µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL6 antibody (Internal) (STJ71725)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

Choose Options

Anti-APOLD1/VERGE antibody (C-Term) (STJ71655)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/FC

Reactivity :

Human

$421.88

Choose Options

Immunofluorescence analysis of paraffin-embedded mouse liver using Apolipoprotein C3 Rabbit monoclonal antibody (STJ11103477) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Apolipoprotein C3 antibody (1-99) [S7MR] (STJ11103477)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

Choose Options

STJ71651 (0. 5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Apolipoprotein F antibody (C-Term) (STJ71651)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

Choose Options

Anti-apolipoprotein L6-Mouse antibody (Internal) (STJ71654)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

$421.88

Choose Options

STJ71656 (0. 5µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Apolipoprotein M-Mouse antibody (Internal) (STJ71656)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Mouse/Rat

$421.88

Choose Options

Confocal imaging of paraffin-embedded Human liver cancer tissue using ApoM Rabbit monoclonal antibody (STJ11102273, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-APOM antibody (1-100) [S3MR] (STJ11102273)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

Choose Options

Immunofluorescence analysis of L929 cells using ApoM Rabbit polyclonal antibody (STJ27289) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-APOM antibody (22-188) (STJ27289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot analysis using ApoM monoclonal antibody against GST-ApoM recombinant protein (1) and human serum (2).

Anti-APOM antibody [10C3G5] (STJ97842)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

Choose Options

Western blot analysis using ApoM monoclonal antibody against human serum (1, 2).

Anti-APOM antibody [8F12C6B8] (STJ97841)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

Choose Options

Western blot analysis using ApoO monoclonal antibody against HepG2 (1) and 3T3L1 (2) cell lysate

Anti-APOO antibody [2F1] (STJ97843)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$194.40 - $469.80

Choose Options

Western blot analysis of SH-SY5Y 293T 3T3 lysis using ApoOL antibody. Antibody was diluted at 1:1000

Anti-APOOL antibody (219-268 aa) (STJ91647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunofluorescence analysis of HeLa cells using APP antibody (STJ112892) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-APP antibody (1-100) (STJ112892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot analysis of extracts of U-87MG cells, using APP antibody (STJ118700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-APP antibody (1-100) (STJ118700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from SKOV3 cells, using APP Rabbit polyclonal antibody (STJ22657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APP antibody (1-100) (STJ22657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

Choose Options

Immunohistochemistry analysis of paraffin-embedded human esophagus using APP antibody (STJ22656) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-APP antibody (200-300) (STJ22656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$151.88 - $708.08

Choose Options

Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-amyloid-Alpha-beta (PTR2007) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SH-SY5YPredicted band size: 86kDaObserved band size: 95kDa

Anti-APP antibody (200-300) [PTR2007] (STJA0006225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunofluorescence analysis of Hela cell. 1, Amyloid-Beta Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-APP antibody (221-270 aa) (STJ98585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-APP antibody (350-450 aa) (STJ13100611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

Choose Options

Anti-APP antibody (400-450 aa) (STJ13100557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

Choose Options

Anti-APP antibody (400-450 aa) (STJ13100558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

Choose Options

Western blot analysis of 3T3 Hela cells using Amyloid-Beta Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-APP antibody (631-680 aa) (STJ98604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Immunocytochemistry analysis of Amyloid Precursor Protein (green) in Hela using Amyloid Precursor Protein antibody, and DAPI (blue).

Anti-APP antibody (66-82 aa) [R09-3F7] (STJA0010881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$332.78

Choose Options

Western blot analysis of Amyloid Precursor Protein in Hela lysates using Amyloid Precursor Protein antibody.

Anti-APP antibody (671-687 aa) [R01-9E9] (STJA0010885)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

$332.78

Choose Options