![Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit polyclonal antibody (STJ11100834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89769/358241/STJ11100834_1__55275.1713122574.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit polyclonal antibody (STJ11100834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit polyclonal antibody (STJ11100834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89769/358241/STJ11100834_1__55275.1713122574.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit polyclonal antibody (STJ11100834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-ANKRD52 antibody (450-660) (STJ11100834)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ANKRD52 (450-660) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ANKRD52 |
| Gene ID | 283373 |
| Uniprot ID | ANR52_HUMAN |
| Immunogen Region | 450-660 |
| Immunogen Sequence | RRRDKFGRTPLHYAAANGSY QCAVTLVTAGAGVNEADCKG CSPLHYAAASDTYRRAEPHT PSSHDAEEDEPLKESRRKEA FFCLEFLLDNGADPSLRDRQ GYTAVHYAAAYGNRQNLELL LEMSFNCLEDVESTIPVSPL HLAAYNGHCEALKTLAETLV NLDVRDHKGRTALFLATERG STECVEVLTAHGASALIKER KRKWTPLHAAA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 450-660 of human ANKRD52 (NP_775866.2). |
Additional Info
| Function | Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. |
| Protein Name | Serine/Threonine-Protein Phosphatase 6 Regulatory Ankyrin Repeat Subunit CPp6-Ars-CSerine/Threonine-Protein Phosphatase 6 Regulatory Subunit Ars-CAnkyrin Repeat Domain-Containing Protein 52 |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase 6 Regulatory Ankyrin Repeat Subunit C antibodyAnti-Pp6-Ars-C antibodyAnti-Serine/Threonine-Protein Phosphatase 6 Regulatory Subunit Ars-C antibodyAnti-Ankyrin Repeat Domain-Containing Protein 52 antibodyAnti-ANKRD52 antibody |
Information sourced from Uniprot.org