Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD27 antibody (1-80 aa) (STJ191672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-CD27 (PT1818) antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: K562Predicted band size: 29kDaObserved band size: 40kDa

Anti-CD27 antibody (160-260) [PT1818] (STJA0006232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$67.62 - $360.18

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STJ73070 (0. 3µg/ml) staining of Human Spleen (A) , Tonsil (B) and Thymus (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD27 antibody (161-175) (STJ73070)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$431.25

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Western blot analysis of lysates from Mouse lung, using CD27 Rabbit polyclonal antibody (STJ118100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD27 antibody (20-191) (STJ118100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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Western blot analysis of extracts of Raji cells, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD27 antibody (20-191) (STJ22984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-CD27 antibody [203-6] (STJ16101136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

$425.73 - $1,862.31

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Anti-CD27 antibody [203-6] (STJ16102761)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F/IF/WB

Reactivity :

Human

$534.75

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Anti-CD27 antibody [5F24] (STJA0027858)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$319.47 - $395.37

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Anti-CD27 antibody [5F24] {APC} (STJA0029338)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$389.85 - $486.45

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Anti-CD27 antibody [5F24] {FITC} (STJA0028372)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$319.47 - $392.61

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Anti-CD27 antibody [5F24] {PerCP} (STJA0030304)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$366.39 - $456.09

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Anti-CD27 antibody [5F24] {PE} (STJA0031270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$389.85 - $486.45

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Anti-CD27 antibody [PT1331R] (STJA0040116)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-CD27 antibody [R01-7E3] (STJA0034135)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Anti-CD27 antibody [R08-8H1] (STJA0037009)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human/Rat

$184.23 - $388.47

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Flow cytometry:1X10^6 293F (Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, left) or CD271/NGFR Rabbit monoclonal antibody (STJ11105254, 2 Mu g/mL, right).

Anti-CD271 antibody (32-254) [S5254RM] (STJ11105254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse/Rat

$174.57 - $765.21

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Anti-CD271 antibody [NGFR5] (STJ16103250)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-P/ICC/MC

Reactivity :

Human/Rabbit/Cat/Non-Human Primates/Ferret

$578.91

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD272 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-CD272 antibody (30-183 aa) [R09-4A1] (STJA0011098)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

$184.23 - $388.47

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Anti-CD272/BTLA antibody [SRM13405] (STJ11108135)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Mouse

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Western blot analysis of lysates from Mouse thymus, using CD274 Rabbit monoclonal antibody (STJ11105258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (19-235) [S5258RM] (STJ11105258)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$163.53 - $719.67

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ113753) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (19-238) (STJ113753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using CD274 antibody (STJ11103333) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD274 antibody (19-239) (STJ11103333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunofluorescence analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ22985) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD274 antibody (190-290) (STJ22985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry of baboon spleen with Anti-CD274 primary antibody (STJ502399) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD274 antibody (80-130) (STJ502399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$552.69

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Anti-CD274 antibody (80-130) {Biotin} (STJ502400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$686.55

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Anti-CD274 antibody (80-130) {FITC} (STJ502401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$686.55

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274/CD274 Rabbit monoclonal antibody (STJ11103966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (ECD) [S3966RM] (STJ11103966)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$174.57 - $765.21

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Anti-CD274 antibody (Extracellular region) [M051] (STJA0005523)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

$544.41

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Anti-CD274 antibody (Extracellular region) [M080] (STJA0005524)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

$544.41

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Anti-CD274 antibody [SRM15057] (STJ11109787)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/FC/ELISA

Reactivity :

Mouse

$0.00

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STJ70646 (0. 01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD274/PD-L1 antibody (C-Term) (STJ70646)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

$431.25

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Biotinylated STJ70646 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-CD274/PD-L1, Biotinylated antibody (C-Term) {Biotin} (STJ73200)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$431.25

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Anti-CD274/PD-L1/B7-H1 antibody (Met1-Arg238) [1A058] (STJA0021896)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$272.55 - $380.19

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Anti-CD274/PD-L1/B7-H1 antibody (Met1-Arg238) [1A059] (STJA0021897)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$272.55 - $380.19

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Anti-CD274/PD-L1/B7-H1 antibody (Met1-Arg238) [1A060] (STJA0021898)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$272.55 - $380.19

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Anti-CD274/PD-L1/B7-H1 antibody (Met1-Arg238) [1A061] (STJA0021899)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$272.55 - $380.19

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Anti-CD274/PD-L1/B7-H1 antibody [4059A11] (STJA0022264)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

$269.79 - $1,820.91

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Anti-CD274/PD-L1/B7-H1 antibody [SAA2003] (STJA0020550)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

$273.93 - $1,615.29

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Anti-CD274/PD-L1/B7-H1 antibody [SAA2018] (STJA0020565)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

$273.93 - $1,615.29

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Anti-CD274/PD-L1/B7-H1 antibody [SP142] (STJA0021671)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

$273.93 - $1,615.29

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Western blot analysis of Mouse spleen, using CD275 Rabbit polyclonal antibody (STJ11105078) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD275 antibody (47-270 aa) (STJ11105078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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Western blot analysis of 293 cells using CD276 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD276 antibody (271-320 aa) (STJ96650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of CD276 in paraffin-embedded human thyroid cancer tissue using CD276 Rabbit polyclonal antibody (STJ11105431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CD276 antibody (29-245) (STJ11105431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of various lysates, using CD276/B7-H3 rabbit polyclonal antibody (STJ119347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : RajiExposure time: 180s.

Anti-CD276 antibody (29-245) (STJ119347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$152.49 - $697.59

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Anti-CD276 antibody (AA range: 100-200) [PTR1401] (STJA0038904)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-CD276 antibody (Extracellular region) [M045] (STJA0005278)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human

$538.89

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Western blot analysis using CD276 monoclonal antibody against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CD276 antibody [6A1] (STJ97917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$205.62 - $496.80

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Anti-CD276 antibody [R02-5J6] (STJA0036434)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/FC

Reactivity :

Human/Mouse

$184.23 - $388.47

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