Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from NIH/3T3 and Jurkat cells, using CCS Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CCS antibody (225-274 aa) (STJ92080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCSAP antibody (94-144 aa) (STJ194311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCSER2 antibody (609-659 aa) (STJ195977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using TCP1 beta/CCT2 Rabbit pAb (STJ28629) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-CCT2 antibody (206-535) (STJ28629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded rat brain using TCP1 beta/CCT2 Rabbit monoclonal antibody (STJ11102134) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CCT2 antibody (436-535) [S4MR] (STJ11102134)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis using TCP-1 Beta monoclonal antibody against HeLa (1) , MCF-7 (2) , Jurkat (3) , T47D (4) , K562 (5) , A431 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-CCT2 antibody [5B5C4] (STJ98415)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Anti-CCT2 antibody [5B5F5] (STJ98414)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of U-2 OS cells using CCT3 antibody (STJ28630) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CCT3 antibody (1-300) (STJ28630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CCT3 antibody (STJ191506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ71930 (0. 01µg/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CCT3/TCP1 antibody (C-Term) (STJ71930)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow/Dog

£312.50

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Immunofluorescence analysis of HeLa cells using CCT4 Rabbit polyclonal antibody (STJ28631) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-CCT4 antibody (130-390) (STJ28631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CCT4 antibody (STJA0007252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CCT5 antibody (STJ28632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCT5 antibody (1-270) (STJ28632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of HepG2 cells using TCP-1 Epsilon Polyclonal Antibody

Anti-CCT5 antibody (241-290 aa) (STJ95949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using TCP-1 Zeta Polyclonal Antibody diluted at 1：500

Anti-CCT6A antibody (171-220 aa) (STJ95950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and CCT6A knockdown (KD) HeLa cells using CCT6A Rabbit polyclonal antibody (STJ11105199) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.

Anti-CCT6A antibody (80-250) (STJ11105199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of extracts of Mouse brain, using CCT6A antibody (STJ29904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CCT6A antibody (80-250) (STJ29904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using CCT6B Rabbit polyclonal antibody (STJ116824) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CCT6B antibody (120-340) (STJ116824)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CCT6B antibody (STJA0009742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of HeLa cells using TCP-1 Eta Polyclonal Antibody

Anti-CCT7 antibody (221-270 aa) (STJ95951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using CCT7 Rabbit polyclonal antibody (STJ114039) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CCT7 antibody (310-500) (STJ114039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded Mouse brain using CCT8 antibody (STJ111219) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CCT8 antibody (248-497) (STJ111219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of HeLa cells using TCP-1 Theta Polyclonal Antibody

Anti-CCT8 antibody (361-410 aa) (STJ95952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-CCT8L1P antibody (465-515 aa) (STJ197658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with Rabbit IgG isotype control (2 Mu g/mL, left) or CD10 Rabbit monoclonal antibody (STJ11105672, 2 Mu g/mL, right) , followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining.

Anti-CD10 antibody (52-750) [S5672RM] (STJ11105672)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Western blot analysis of CD10 at dilution of 1:500

Anti-CD10 antibody [SM0418] (STJA0010418)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-CD10 antibody [SN5c] (STJ16101902)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IP/WB

Reactivity :

Human

£741.50

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Western blot analysis of lysates from 293T-CD101 cells, using CD101 Rabbit monoclonal antibody (STJ11105218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD101 antibody (21-410 aa) [S5218RM] (STJ11105218)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD101, using CD101 Rabbit polyclonal antibody (STJ11105359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD101 antibody (21-410) (STJ11105359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of mouse brain cells using CD101 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-CD101 antibody (731-780 aa) (STJ97295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using CD102 antibody (STJ11104966) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD102 antibody (20-222 aa) (STJ11104966)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-CD102 antibody [B-T1] (STJ16100514)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/IP

Reactivity :

Human

£741.50

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Anti-CD102 antibody [SRM] (STJ11106975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of Mouse thymus, using CD103 Rabbit polyclonal antibody (STJ11104994) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CD103 antibody (191-385 aa) (STJ11104994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-CD104 antibody [450-9D] (STJ16100525)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/FC/IHC-F/IF

Reactivity :

Human/Ferret/Mink/Mustelid

£741.50

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Western blot analysis of various lysates, using CD105 Rabbit polyclonal antibody (STJ11105355) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :NIH/3T3Exposure time: 5s.

Anti-CD105 antibody (27-581) (STJ11105355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-CD105 antibody [MEM-226] (STJ16100186)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Rat/Human

£431.50

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Western blot analysis of CD105 at dilution of 1:1000

Anti-CD105 antibody [SM4908] (STJA0004908)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse

£170.50 - £233.50

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Western blot analysis of various lysates, using CD106 Rabbit polyclonal antibody (STJ11105075) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD106 antibody (25-310 aa) (STJ11105075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of various lysates, using CD107b Rabbit polyclonal antibody (STJ11105072) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD107b antibody (26-189 aa) (STJ11105072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-CD108 antibody [MEM-150] (STJ16100094)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Human

£431.50

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Western blot analysis of various lysates, using CD109 Rabbit monoclonal antibody (STJ11105222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :K-562Exposure time: 60s.

Anti-CD109 antibody (223-462 aa) [S5222RM] (STJ11105222)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates, using CD112 Rabbit polyclonal antibody (STJ11105181) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD112 antibody (32-345 aa) (STJ11105181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-CD115/Csf1r antibody [AFS98] (STJA0001689)

Clonality :

Monoclonal

Host :

Rat

Applications :

Block/Depletion/Neut/FC/WB

Reactivity :

Mouse

£93.50 - £188.50

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Western blot analysis of extracts of HeLa cells, using CD117 antibody (STJ11104271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD117 antibody (25-523) (STJ11104271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of extracts from mouse lung, using CD117/c-Kit Rabbit monoclonal antibody (STJ11105091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD117/c-Kit antibody [S5091RM] (STJ11105091)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of EL4, using CD119 Rabbit polyclonal antibody (STJ11105176) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD119 antibody (26-250 aa) (STJ11105176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-CD11a antibody [87-6F9] (STJ16100022)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/FUNC/WB/IHC

Reactivity :

Human

£287.50 - £1,324.50

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