Anti-CD11a antibody [87-6F9] (STJ16100022)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2bk
ImmunogenA BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: SP2/0
General Information
| Short Description | Mouse monoclonal anti-CD11a for use in FC, FUNC, WB and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/FUNC/WB/IHC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 87-6F9 |
| Isotype | IgG2bk |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 ยตg/mL, 500 ยตg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow cytometry (0, 5-1, 0 ยตg/million cells in 0, 1 ml). Functional studies (0, 02-2, 0 ยตg/ml without azide). Immunoblotting (1-2 ยตg/ml). Immunohistology (1-2 ยตg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fix |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8ยฐC upon receipt. |
Target Information
| Gene Symbol | ITGAL |
| Gene ID | 3683 |
| Uniprot ID | ITAL_HUMAN |
| Immunogen | A BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: SP2/0 |
Additional Info
| Tissue Specificity | Leukocytes. |
| Post Translational Modifications | In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. |
| Function | Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R. Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15.the interaction is mediated by ITGAL. Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Acts as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion. Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages. |
| Protein Name | Integrin Alpha-LCd11 Antigen-Like Family Member ALeukocyte Adhesion Glycoprotein Lfa-1 Alpha ChainLfa-1aLeukocyte Function-Associated Molecule 1 Alpha ChainCd Antigen Cd11a |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-6798695Reactome: R-HSA-8949275 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinUpon Antigen Recognition By The TcrIs Recruited To Lipid Rafts |
| Alternative Antibody Names | Anti-Integrin Alpha-L antibodyAnti-Cd11 Antigen-Like Family Member A antibodyAnti-Leukocyte Adhesion Glycoprotein Lfa-1 Alpha Chain antibodyAnti-Lfa-1a antibodyAnti-Leukocyte Function-Associated Molecule 1 Alpha Chain antibodyAnti-Cd Antigen Cd11a antibodyAnti-ITGAL antibodyAnti-CD11A antibody |
Information sourced from Uniprot.org