• Western blot analysis of extracts from mouse lung, using CD117/c-Kit Rabbit monoclonal antibody (STJ11105091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD117/c-Kit antibody [S5091RM] (STJ11105091)

SKU:
STJ11105091

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD117/c-Kit is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5091RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Kit
Gene ID: 16590
Uniprot ID: KIT_MOUSE
Immunogen: Recombinant protein of mouse CD117/c-Kit.
Immunogen Sequence: SQPSASPGEPSPPSIHPAQS ELIVEAGDTLSLTCIDPDFV RWTFKTYFNEMVENKKNEWI QEKAEATRTGTYTCSNSNGL TSSIYVFVRDPAKLFLVGLP LFGKEDSDALVRCPLTDPQV SNYSLIECDGKSLPTDLTFV PNPKAGITIKNVKRAYHRLC VRCAAQRDGTWLHSDKFTLK VRAAIKAIPVVSVPETSHLL KKGDTFTVVCTIKDVSTSVN SMWLKMNPQPQHIAQVKHN
Tissue Specificity Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.
Post Translational Modifications Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation. Autophosphorylated on tyrosine residues. KITLG/SCF binding promotes autophosphorylation of isoform 1 and isoform 2. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF, while isoform 2 requires stimulation by KITLG/SCF for phosphorylation (in vitro). Phosphorylation of Tyr-573 is required for interaction with PTPN6/SHP-1. Phosphorylation of Tyr-571 is required for interaction with PTPN11/SHP-2. Phosphorylated tyrosine residues are important for interaction with specific binding partners.
Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
Protein Name Mast/Stem Cell Growth Factor Receptor Kit
Scfr
Proto-Oncogene C-Kit
Tyrosine-Protein Kinase Kit
Cd Antigen Cd117
Database Links Reactome: R-MMU-1257604
Reactome: -MMU-1433557
Reactome: -MMU-1433559
Reactome: -MMU-5673001
Reactome: -MMU-6811558
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cell Membrane
Isoform 3: Cytoplasm
Detected In The Cytoplasm Of Spermatozoa
Especially In The Equatorial And Subacrosomal Region Of The Sperm Head
Alternative Antibody Names Anti-Mast/Stem Cell Growth Factor Receptor Kit antibody
Anti-Scfr antibody
Anti-Proto-Oncogene C-Kit antibody
Anti-Tyrosine-Protein Kinase Kit antibody
Anti-Cd Antigen Cd117 antibody
Anti-Kit antibody
Anti-Sl antibody

Information sourced from Uniprot.org

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