Western Blot Antibodies | St John's Laboratory

Western blot analysis of various lysates, using CD34 Rabbit monoclonal antibody (STJ11105019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CD34 antibody [S5019RM] (STJ11105019)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Anti-CD34 antibody [SRM] (STJ11106962)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-CD35 antibody (1990-2038 aa) (STJ13100968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of A549 3T3 mouse-liver mouse-brain cells using CD35 Polyclonal Antibody diluted at 1:800. Secondary antibody was diluted at 1:20000

Anti-CD35 antibody (300-350 & 740-790 aa) (STJ98580)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CD35 antibody [E11] (STJ16101912)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IHC-P/WB

Reactivity :

Human

£741.50

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Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (STJ28356) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD36 antibody (30-439) (STJ28356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (STJ11101554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-CD36 antibody (300-400) [S4MR] (STJ11101554)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from U-937 cells using CD36/SR-B3 Rabbit polyclonal antibody (STJ22993) at 1:700 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-CD36 antibody (301-400) (STJ22993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of MCF7, 4T1 cells using CD36 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD36 antibody (331-380 aa) (STJ97280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Tilapia

£48.00 - £252.00

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Immunofluorescence analysis of U937 cells using CD36/SR-B3 Rabbit polyclonal antibody (STJ116914) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD36 antibody (50-150) (STJ116914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using CD36/SR-B3 Rabbit pAb (STJ119465) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-CD36 antibody (50-150) (STJ119465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD36 antibody (ECD) (STJ13100091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0. 01M containing Triton X 0. 005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to extracellular domain of human CD36 (Fatty acid translocase) : IgG (STJ13100092) diluted 1: 100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1: 100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Anti-CD36 antibody (ECD) (STJ13100092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-CD36 antibody (ECD) (STJ13100102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Marmoset/Rat/Mouse

£331.50

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Anti-CD36 antibody (ECD) (STJ13100509)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Human/Marmoset/Rat/Mouse

£331.50

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Anti-CD36 antibody (ECD) (STJ13100903)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-CD36 antibody (STJ500463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human

£427.50

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Anti-CD36 antibody {Biotin} (STJ500464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human

£528.50

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Anti-CD36 antibody {FITC} (STJ500465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human

£528.50

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Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (STJ11102904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CD36/SR-B3 antibody (93-165) (STJ11102904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD364/PI16 using CD364/PI16 Rabbit mAb (STJ11107371) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 20 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( Exposure time: 10s.

Anti-CD364/PI16 antibody (30-210 aa) [SRM] (STJ11107371)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Anti-CD37 antibody (112-241 aa) (STJ11106006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of HeLa cells using CD37 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD37 antibody (81-130 aa) (STJ96827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CD38 Rabbit pAb (STJ22994) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-CD38 antibody (181-300) (STJ22994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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THP-1 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD38 (ABT-CD38) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: THP-1Predicted band size: 34kDaObserved band size: 40kDa

Anti-CD38 antibody (200-300 aa) [ABT-CD38] (STJ196916)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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THP1 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD38 (ABT298) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: THP1Predicted band size: 34kDaObserved band size: 40kDa

Anti-CD38 antibody (200-300 aa) [ABT298] (STJ197120)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of 293 cells using CD38 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CD38 antibody (211-260 aa) (STJ96757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ73361 (2µg/ml) staining of Human Spinal Cord cancer lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD38 antibody (226-237) (STJ73361)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Immunofluorescence analysis of THP-1 cells using CD38 Rabbit polyclonal antibody (STJ115571) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD38 antibody (43-300) (STJ115571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD38 antibody (45-304 aa) [SRM] (STJ11107232)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis using CD38 monoclonal antibody against CD38-hIgGFc transfected HEK293 cell lysate.

Anti-CD38 antibody [6E12D] (STJ97924)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-CD39 antibody (380-430) (STJ500891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

£427.50

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Anti-CD39 antibody (380-430) {Biotin} (STJ500889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

£528.50

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Anti-CD39 antibody (380-430) {FITC} (STJ500890)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

£528.50

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Anti-CD39 antibody [AC2.5] (STJ16102205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F/IP/WB

Reactivity :

Human

£636.50

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Western blot analysis of various lysates, using CD3d Rabbit polyclonal antibody (STJ11105242) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CD3d antibody (15-115 aa) (STJ11105242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunofluorescence analysis of Jurkat cells using CD3D antibody (STJ22995) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CD3D antibody (22-105) (STJ22995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of HeLa cells using CD3-Delta Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD3D antibody (41-90 aa) (STJ96823)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using CD3-Delta Polyclonal Antibody

Anti-CD3D antibody (7-56 aa) (STJ92125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, using CD3D Rabbit mAb (STJ11102545) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CD3D antibody (72-171) [S5MR] (STJ11102545)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ73090 (1µg/ml) staining of Jurkat (A) and Molt4 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD3D-Isoform a antibody (Internal) (STJ73090)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Anti-CD3E antibody (1-100 aa) [ABT-CD3] (STJ196937)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Confocal immunofluorescent analysis of Jurkat cells labeling CD3 (ABT295) Mouse monoclonal antibody followed by Goat Anti- mouse IgG H&L (FITC Conjugate) secondary antibody (green). Counterstained with DAPI (blue). Negative control: PBS was used to instead of primary antibody, followed by Goat Anti- mouse IgG H&L (FITC Conjugate) secondary antibody (green).

Anti-CD3E antibody (1-100 aa) [ABT295] (STJ197119)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/ICC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of RAW264. 7 cells using CD3E Antigen antibody (STJ114289) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CD3E antibody (111-207) (STJ114289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Jurkat cells, using CD3E antibody (STJ22996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD3E antibody (111-207) (STJ22996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-CD3E Antibody (STJ500448) of rat thymus at 1:250 dilution in buffer.

Anti-CD3E antibody (150-200) (STJ500448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CD3E antibody (150-200) {Biotin} (STJ500454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CD3E antibody (150-200) {FITC} (STJ500455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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