Anti-Calreticulin antibody [R03-9K4] (STJA0035896)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Calreticulin-ER Marker
General Information
| Short Description | Rabbit monoclonal anti-Calreticulin for use in WB, IHC-P, ICC, IF, IP and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/IP/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R03-9K4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CALR |
| Gene ID | 811 |
| Uniprot ID | CALR_HUMAN |
| Immunogen | A synthesized peptide derived from human Calreticulin-ER Marker |
Additional Info
| Function | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy. |
| Protein Name | CalreticulinCrp55CalregulinEndoplasmic Reticulum Resident Protein 60Erp60HacbpGrp60 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-168316Reactome: R-HSA-3000480Reactome: R-HSA-3000484Reactome: R-HSA-381183Reactome: R-HSA-901042Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic Reticulum LumenCytoplasmCytosolSecretedExtracellular SpaceExtracellular MatrixCell SurfaceSarcoplasmic Reticulum LumenCytoplasmic VesicleSecretory VesicleCortical GranuleCytolytic GranuleAlso Found In Cell Surface (T Cells)Cytosol And Extracellular MatrixDuring Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical GranulesIn Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The CortexIn Cortical Granules Of Non-Activated OocytesIs Exocytosed During The Cortical Reaction In Response To Oocyte Activation |
| Alternative Antibody Names | Anti-Calreticulin antibodyAnti-Crp55 antibodyAnti-Calregulin antibodyAnti-Endoplasmic Reticulum Resident Protein 60 antibodyAnti-Erp60 antibodyAnti-Hacbp antibodyAnti-Grp60 antibodyAnti-CALR antibodyAnti-CRTC antibody |
Information sourced from Uniprot.org