Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from Mouse lung, using CD27 Rabbit polyclonal antibody (STJ118100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD27 antibody (20-191) (STJ118100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of extracts of Raji cells, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD27 antibody (20-191) (STJ22984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD27 antibody [203-6] (STJ16101136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Flow cytometry:1X10^6 293F (Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, left) or CD271/NGFR Rabbit monoclonal antibody (STJ11105254, 2 Mu g/mL, right).

Anti-CD271 antibody (32-254) [S5254RM] (STJ11105254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD272 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-CD272 antibody (30-183 aa) [R09-4A1] (STJA0011098)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£246.50

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Western blot analysis of lysates from Mouse thymus, using CD274 Rabbit monoclonal antibody (STJ11105258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (19-235) [S5258RM] (STJ11105258)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ113753) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (19-238) (STJ113753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CD274 antibody (STJ11103333) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD274 antibody (19-239) (STJ11103333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of HepG2 cells, using PD-L1/CD274 antibody (STJ11104347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (190-290) (STJ11104347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ22985) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD274 antibody (190-290) (STJ22985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry of baboon spleen with Anti-CD274 primary antibody (STJ502399) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD274 antibody (80-130) (STJ502399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CD274 antibody (80-130) {Biotin} (STJ502400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-CD274 antibody (80-130) {FITC} (STJ502401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274/CD274 Rabbit monoclonal antibody (STJ11103966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (ECD) [S3966RM] (STJ11103966)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-CD274 antibody (Extracellular region) [M051] (STJA0005523)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£394.50

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Anti-CD274 antibody (Extracellular region) [M080] (STJA0005524)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£394.50

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Anti-CD274 antibody [SMM] (STJ11106420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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STJ70646 (0. 01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD274/PD-L1 antibody (C-Term) (STJ70646)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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Biotinylated STJ70646 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-CD274/PD-L1, Biotinylated antibody (C-Term) {Biotin} (STJ73200)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of Mouse spleen, using CD275 Rabbit polyclonal antibody (STJ11105078) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD275 antibody (47-270 aa) (STJ11105078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of 293 cells using CD276 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD276 antibody (271-320 aa) (STJ96650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of CD276 in paraffin-embedded human thyroid cancer tissue using CD276 Rabbit polyclonal antibody (STJ11105431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CD276 antibody (29-245) (STJ11105431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates, using CD276/B7-H3 rabbit polyclonal antibody (STJ119347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : RajiExposure time: 180s.

Anti-CD276 antibody (29-245) (STJ119347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CD276 antibody (Extracellular region) [M045] (STJA0005278)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human

£390.50

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Western blot analysis using CD276 monoclonal antibody against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CD276 antibody [6A1] (STJ97917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various lysates, using CD276/B7-H3 Rabbit monoclonal antibody (STJ11105227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : RajiExposure time: 90s.

Anti-CD276/B7-H3 antibody [S5227RM] (STJ11105227)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-CD279/Pdcd1 antibody [29F.1A12] (STJA0001817)

Clonality :

Monoclonal

Host :

Rat

Applications :

Block/Neut/FC/WB/IHC/IF

Reactivity :

Mouse

£93.50 - £188.50

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Anti-CD279/Pdcd1 antibody [29F.1A12] (STJA0001818)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/WB/IHC/IF

Reactivity :

Mouse

£93.50 - £114.50

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Western blot analysis of K562 cells using CD28 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD28 antibody (11-60 aa) (STJ96609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Flow cytometric analysis of CD28 Rabbit monoclonal antibody Cocotail (10 Mu g/mL) in Jurkat cells (Orange) compare to Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) Isotype control (Blue) and non-staining control (Red).

Anti-CD28 antibody (Full Length) [S5MR] (STJ11103275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-CD28 antibody (Ig-V-like Region) (STJA0005685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

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STJ72503 (0. 3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD28 antibody (Internal) (STJ72503)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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STJ72504 (0. 3µg/ml) staining of Mouse Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Cd28-Mouse antibody (Internal) (STJ72504)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

£312.50

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Anti-CD29 antibody [MEM-101A] (STJ16100040)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Dog/Pig/Human

£405.50

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Anti-CD294 antibody (1-32 aa) (STJ11107024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-CD298/ATP1B3 antibody (180-279 aa) [SRM] (STJ11106584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD299/DC-SIGNR using CD299/DC-SIGNR Rabbit mAb (STJ11107384) at 1:3000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 20 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( Exposure time: 180s.

Anti-CD299/DC-SIGNR antibody (71-399 aa) [SRM] (STJ11107384)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Anti-CD2AP antibody (250-300 aa) (STJ13100972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£331.50

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Anti-CD2AP antibody (320-400 aa) (STJ191673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CD2AP Rabbit polyclonal antibody (STJ110098) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Cd2ap antibody (360-639) (STJ110098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD2AP antibody (50-100 aa) (STJ13100971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£331.50

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Anti-CD2AP antibody (560-630 aa) (STJ13100970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of lysates from wild type (WT) and CD2BP2 knockout (KO) 293T cells, using [KO Validated] CD2BP2 Rabbit polyclonal antibody (STJ11100767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD2BP2 antibody (1-341) (STJ11100767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using CD2BP2 Rabbit polyclonal antibody (STJ118222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD2BP2 antibody (1-341) (STJ118222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using CD2BP2 Polyclonal Antibody diluted at 1：1000

Anti-CD2BP2 antibody (101-150 aa) (STJ92111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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STJ70564 (0. 3µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD2BP2 antibody (N-Term) (STJ70564)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of CD3 at dilution of 1:1000

Anti-CD3 antibody [SM0382] (STJA0010382)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Anti-CD3 Epsilon antibody [145-2C11] (STJA0001659)

Clonality :

Monoclonal

Host :

Armenian Hamster

Applications :

Activ/Depletion/FC/WB/IHC/IF/IP

Reactivity :

Mouse

£93.50 - £188.50

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