Immunohistochemistry Antibodies | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using EP300 rabbit polyclonal antibody (STJ114983) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-EP300 antibody (1-270) (STJ114983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of EP300 in paraffin-embedded mouse spleen tissue using EP300 Rabbit polyclonal antibody (STJ11105314) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-EP300 antibody (940-1030) (STJ11105314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, p300 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-EP300 antibody (Internal) (STJ97645)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-EP400 antibody (2057-2107 aa) (STJ196621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry of mouse liver with Anti-EPAC2 primary antibody (STJ500902) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 20X.

Anti-EPAC2 antibody (743-762) (STJ500902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Horse/Human/Mouse/Rat

£466.50

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Anti-EPAC2 antibody (743-762) {Biotin} (STJ500907)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Horse/Human/Mouse/Rat

£528.50

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Anti-EPAC2 antibody (743-762) {FITC} (STJ500908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Horse/Human/Mouse/Rat

£528.50

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Western blot analysis of various lysates using EPAS1/HIF2 Alpha Rabbit pAb (STJ29691) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EPAS1 antibody (678-745) (STJ29691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human-stomach tissue. 1, EPAS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EPAS1 antibody (STJ96690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using 4.1G Polyclonal Antibody

Anti-EPB41L2 antibody (530-610 aa) (STJ91381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-EPB41L2/4.1G antibody (347-357) (STJ71367)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

£312.50

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STJ71353 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-EPB41L2/4.1G antibody (593-604) (STJ71353)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/FC

Reactivity :

Human

£312.50

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Anti-EPB41L4B/EHM2 antibody (Internal) (STJ71354)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using EpCAM rabbit monoclonal antibody (STJ11101274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-EPCAM antibody (1-100) [S4MR] (STJ11101274)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-EpCAM (ABT-EPCAM) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: SW480 Lane 3: HepG2Predicted band size: 35kDaObserved band size: 42kDa

Anti-EPCAM antibody (100-200 aa) [ABT-EPCAM] (STJ196761)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis using EP-CAM monoclonal antibody against HTC116 (1) and T47D (2) cell lysate.

Anti-EPCAM antibody [7E11] (STJ98021)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Human colon adenocarcinoma stained with Ber-EP4 antibody using peroxidase-conjugate and DAB chromogen. Note the membranous staining of tumor cells

Anti-EPCAM antibody [Ber‐EP4] (STJ180093)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£164.50 - £601.50

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Anti-EPCAM antibody [BS14] (STJ16101361)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Dog/Rat/Pig/Sheep

£1,229.50

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Anti-EpCAM antibody [SM5025] (STJA0005025)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of EpCAM. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-EpCAM antibody [SM5087] (STJA0005087)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Anti-EPCAM antibody [VU-1D9] (STJ16100748)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Cow/Human

£287.50 - £1,324.50

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Anti-EPDR1 antibody (STJA0004876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200

Anti-EPG5 antibody (141-190 aa) (STJ98818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using EphA1 Polyclonal Antibody

Anti-EPHA1 antibody (541-590 aa) (STJ92938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using EphA1 monoclonal antibody against A549 (1) and HeLa (2) cell lysate.

Anti-EPHA1 antibody [5D2] (STJ98022)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using EphA2 monoclonal antibody against NIH/3T3 cell lysate.

Anti-EPHA2 antibody [1B3C7] (STJ98024)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemistry analysis of paraffin-embedded Rat kidney using EPHA3/EPHA4/EPHA5 pAb (STJ11106609) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-EPHA3/EPHA4/EPHA5 antibody (700-800 aa) (STJ11106609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of COS7 cells, using EPHA6 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EPHA6 antibody (891-940 aa) (STJ92945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using EphA7 monoclonal antibody against truncated GST-EphA7 recombinant protein (1) and truncated EphA7 (aa25-556) -hIgGFc transfected CHOK1 cell lysate (2).

Anti-EPHA7 antibody (27-210 aa) [6C8G7] (STJ98029)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of 293T-UV cells using EphA7 Polyclonal Antibody diluted at 1：500

Anti-EPHA7 antibody (451-500 aa) (STJ92946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot with Anti-EPHA7 Antibody (STJ500924) of SKNMC cell at 1:500 dilution in buffer.

Anti-EPHA7 antibody (500-550) (STJ500924)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-EPHA7 antibody (500-550) {Biotin} (STJ500925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-EPHA7 antibody (500-550) {FITC} (STJ500926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis using EphB1 monoclonal antibody against MDA-MB-468 (1) , MDA-MB-453 (2) , MCF-7 (3) , T47D (4) and SKBR-3 (5) cell lysate.

Anti-EPHB1 antibody (19-133 aa) [5F10A4] (STJ98031)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using EphB1/2 Polyclonal Antibody diluted at 1：500

Anti-EphB1/2 antibody (561-610 aa) (STJ92948)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells, using EPHB1/2/3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EphB1/2/3 antibody (631-680 aa) (STJ92949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 3T3 cells using EphB1/2/3/4 Polyclonal Antibody

Anti-EphB1/2/3/4 antibody (566-615 aa) (STJ92950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using EphB2 Polyclonal Antibody diluted at 1：1000

Anti-EPHB2 antibody (991-1040 aa) (STJ92951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using EphB3 monoclonal antibody against truncated EphB3-His recombinant protein.

Anti-EPHB3 antibody (39-212 aa) [4A122D1] (STJ98033)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemical analysis of paraffin-embedded Human-breast, antibody was diluted at 1:100

Anti-EPHB3 antibody (650-700 aa) (STJ98982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Eph receptor B4/HTK antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EPHB4 antibody (17-529 aa) [R04-3B0] (STJA0012363)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis of HY926 cells using EphB4 Polyclonal Antibody diluted at 1：2000

Anti-EPHB4 antibody (571-620 aa) (STJ92952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis using EphB4 monoclonal antibody against truncated EphB4 recombinant protein.

Anti-EPHB4 antibody [5B8F7] (STJ98034)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using EphB6 monoclonal antibody against truncated EhpB6 recombinant protein (1).

Anti-EPHB6 antibody (601-750 aa) [2A6B9] (STJ98038)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using EphB6 Polyclonal Antibody

Anti-EPHB6 antibody (861-910 aa) (STJ92953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ73642 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-EPHB6 antibody (Internal) (STJ73642)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Western blot analysis using EphB6 monoclonal antibody against Jurkat (1) and NIH/3T3 (2) cell lysate.

Anti-EPHB6 antibody [8E7H12] (STJ98037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of lysates from HepG2 cells, using Ephrin A1 Rabbit mAb (STJ11103650) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Ephrin A1 antibody (1-100) [S0MR] (STJ11103650)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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