• Western blot analysis of Jurkat, using CHI3L2 rabbit polyclonal antibody (STJ29968) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human liver using CHI3L2 rabbit polyclonal antibody (STJ29968) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CHI3L2 antibody (27-390) (STJ29968)

SKU:
STJ29968

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHI3L2 (27-390) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHI3L2
Gene ID: 1117
Uniprot ID: CH3L2_HUMAN
Immunogen Region: 27-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-390 of human CHI3L2 (NP_003991.2).
Immunogen Sequence: YKLVCYFTNWSQDRQEPGKF TPENIDPFLCSHLIYSFASI ENNKVIIKDKSEVMLYQTIN SLKTKNPKLKILLSIGGYLF GSKGFHPMVDSSTSRLEFIN SIILFLRNHNFDGLDVSWIY PDQKENTHFTVLIHELAEAF QKDFTKSTKERLLLTAGVSA GRQMIDNSYQVEKLAKDLDF INLLSFDFHGSWEKPLITGH NSPLSKGWQDRGPSSYYNVE YAVGYWIHKGMPSEKVVMG
Tissue Specificity Highest expression in chondrocytes, followed by synoviocytes, lung and heart. Not detected in spleen, pancreas, and liver. May also be expressed in developing brain and placenta.
Function Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.
Protein Name Chitinase-3-Like Protein 2
Chondrocyte Protein 39
Ykl-39
Cellular Localisation Secreted
Alternative Antibody Names Anti-Chitinase-3-Like Protein 2 antibody
Anti-Chondrocyte Protein 39 antibody
Anti-Ykl-39 antibody
Anti-CHI3L2 antibody

Information sourced from Uniprot.org

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