Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of COS7 cells, using PDGFR beta Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PDGFRB antibody (991-1040 aa) (STJ95003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of U-87MG cells using 3 Mu g PDHA1 antibody (STJ11104401). Western blot was performed from the immunoprecipitate using PDHA1 antibody (STJ11104401) at a dilution of 1:1000.

Anti-PDHA1 antibody (30-390) (STJ11104401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using PDHA1 Rabbit polyclonal antibody (STJ119549) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PDHA1 antibody (30-390) (STJ119549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of PDHA1 in paraffin-embedded human thyroid cancer using PDHA1 Rabbit polyclonal antibody (STJ24939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PDHA1 antibody (30-390) (STJ24939)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PDHA1 Polyclonal Antibody diluted at 1：2000

Anti-PDHA1 antibody (314-363 aa) (STJ95006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of C2C12 cells using PDHA1 Rabbit monoclonal antibody (STJ11101429, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PDHA1 antibody (50-150) [S9MR] (STJ11101429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis using PDH-E1 Alpha monoclonal antibody against A549, HeLa cell lysate.

Anti-PDHA1 antibody (C-Term) (STJ98528)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Dog/Pig

£144.00 - £348.00

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Western blot analysis of various lysates using PDHA2 Rabbit pAb (STJ111984) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-PDHA2 antibody (119-388) (STJ111984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using PDHA2 Rabbit polyclonal antibody (STJ117192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Pdha2 antibody (230-391) (STJ117192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using PDIA2 Rabbit polyclonal antibody (STJ114937) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PDIA2 antibody (326-525) (STJ114937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of A549, HT29, K562 cells using ERp57 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PDIA3 antibody (111-160 aa) (STJ96585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse brain using ERp57 antibody (STJ24942) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PDIA3 antibody (206-505) (STJ24942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Endogenous ERP57-endoplasmic reticulum lumen marker detected at 1:500 dilution; lysates at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PDIA3 antibody (300aa C-Term) (STJ140035)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Anti-PDIA3 antibody (300aa C-Term) (STJ140036)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Immunofluorescence analysis of PC-3 cells using PDIA4 Rabbit polyclonal antibody (STJ11105691) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDIA4 antibody (200-300) (STJ11105691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of HeLa cells using PDIA6 Rabbit monoclonal antibody (STJ11102028, dilution 1:100) (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-PDIA6 antibody (1-100) [S8MR] (STJ11102028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from wild type (WT) and PDIA6 knockout (KO) 293T cells, using [KO Validated] PDIA6 Rabbit polyclonal antibody (STJ11100065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PDIA6 antibody (20-300) (STJ11100065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of K562 cells using PDIK1L Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PDIK1L antibody (101-150 aa) (STJ95007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using PDK1/PDHK1 Rabbit polyclonal antibody (STJ24945) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDK1 antibody (337-436) (STJ24945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using PDK1 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , Jurkat (3) , HepG2 (4) , PC-12 (5) , and Cos7 (6) cell lysate.

Anti-PDK1 antibody [4A11] (STJ98315)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of U2OS cells using PDK2 antibody (STJ117807) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PDK2 antibody (128-407) (STJ117807)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PDK2 Polyclonal Antibody diluted at 1：500

Anti-PDK2 antibody (260-309 aa) (STJ95011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Human thyroid tissue using PDK3 Rabbit polyclonal antibody (STJ11105596) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-PDK3 antibody (307-406) (STJ11105596)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using PDK4 Rabbit polyclonal antibody (STJ115300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDK4 antibody (102-411) (STJ115300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using PDLIM1/CLP36 antibody (STJ28769). Blue: DAPI for nuclear staining.

Anti-PDLIM1 antibody (1-329) (STJ28769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using CLP36 Polyclonal Antibody diluted at 1：1000

Anti-PDLIM1 antibody (61-110 aa) (STJ92344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using PDLIM1/CLP36 Rabbit monoclonal antibody (STJ11102956) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDLIM1/CLP36 antibody (200-300) [S6MR] (STJ11102956)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of PC-12 cells using PDLIM4 Rabbit polyclonal antibody (STJ11103318) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDLIM4 antibody (80-210) (STJ11103318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ70495 staining (0. 2µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-PDLIM4/RIL antibody (C-Term) (STJ70495)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Immunofluorescence analysis of L929 cells using PDP1 Rabbit polyclonal antibody (STJ116756) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PDP1 antibody (72-260) (STJ116756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of C6 cells using PDP2 Rabbit polyclonal antibody (STJ119385) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PDP2 antibody (67-250) (STJ119385)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from MDA-MB-435 cells, treated with EGF, using PDK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PDPK1 antibody (210-259 aa) (STJ95010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using PDRG1 Polyclonal Antibody

Anti-PDRG1 antibody (58-107 aa) (STJ95013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using PDXDC1 Polyclonal Antibody

Anti-PDXDC1 antibody (677-726 aa) (STJ95015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using PDXK antibody (STJ28770). Blue: DAPI for nuclear staining.

Anti-PDXK antibody (1-312) (STJ28770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1000 dilution. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.

Anti-PDXK antibody (213-312 aa) (STJ11106979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using PDXP Rabbit polyclonal antibody (STJ119571) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PDXP antibody (C-Term) (STJ119571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells, using PDZD2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PDZD2 antibody (51-100 aa) (STJ95016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using PEA-15 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PEA15 antibody (81-130 aa) (STJ95018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunoprecipitation analysis of 150 Mu g extracts of MCF-7 cells using 3 Mu g PEBP1 antibody (STJ24954). Western blot was performed from the immunoprecipitate using PEBP1 antibody (STJ24954) at a dilution of 1:500.

Anti-PEBP1 antibody (1-187) (STJ24954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using RKIP Polyclonal Antibody

Anti-PEBP1 antibody (105-154 aa) (STJ95515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using PEBP4 Rabbit polyclonal antibody (STJ119940) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PEBP4 antibody (90-242) (STJ119940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PECAM-1 antibody [BV8] (STJ16100122)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA/IF/IP

Reactivity :

Human

£662.50

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Anti-PECAM-1 antibody [MEC7.46] (STJ16100083)

Clonality :

Monoclonal

Host :

Rat

Applications :

IHC-F/IHC-P/ELISA/FC/IF/IP

Reactivity :

Mouse

£525.50

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Human appendix tissue was stained with Anti-CD31 (ABT-CD31) Antibody

Anti-PECAM1 antibody (100-200 aa) [ABT-CD31] (STJ196788)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of extracts of THP-1 cells, using CD31/PECAM1 antibody (STJ114880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PECAM1 antibody (630-738) (STJ114880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of human placenta using CD31/PECAM1 Rabbit polyclonal antibody (STJ24956) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-PECAM1 antibody (630-738) (STJ24956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using CD31/PECAM1 rabbit monoclonal antibody (STJ11102190) at 1:60000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PECAM1 antibody (630-738) [S0MR] (STJ11102190)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

Choose Options