• Immunofluorescence analysis of L929 cells using PDHA2 Rabbit polyclonal antibody (STJ117192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PDHA2 Rabbit polyclonal antibody (STJ117192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Pdha2 antibody (230-391) (STJ117192)

SKU:
STJ117192

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDHA2 (230-391) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Pdha2
Gene ID: 18598
Uniprot ID: ODPAT_MOUSE
Immunogen Region: 230-391
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-391 of mouse PDHA2 (NP_032837.1).
Immunogen Sequence: MGTSNERSAASTDYHKKGFI IPGLRVNGMDILCVREATKF AADHCRSGKGPIVMELQTYR YHGHSMSDPGISYRSREEVH NVRSKSDPIMLLRERIISNN LSNIEELKEIDADVKKEVED AAQFATTDPEPAVEDIANYL YHQDPPFEVRGAHKWLKYKS HS
Tissue Specificity Testis.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Pyruvate Dehydrogenase E1 Component Subunit Alpha - Testis-Specific Form - Mitochondrial
Pdhe1-A Type Ii
Database Links Reactome: R-MMU-204174
Reactome: -MMU-389661
Reactome: -MMU-5362517
Reactome: -MMU-70268
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Testis-Specific Form - Mitochondrial antibody
Anti-Pdhe1-A Type Ii antibody
Anti-Pdha2 antibody
Anti-Pdha-2 antibody
Anti-Pdhal antibody

Information sourced from Uniprot.org

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