• Immunofluorescence analysis of PC-3 cells using PDIA4 Rabbit polyclonal antibody (STJ11105691) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using PDIA4 Rabbit polyclonal antibody (STJ11105691) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PDIA4 Rabbit polyclonal antibody (STJ11105691) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PDIA4 Rabbit polyclonal antibody (STJ11105691) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using PDIA4 Rabbit polyclonal antibody (STJ11105691) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PDIA4 antibody (STJ11105691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using PDIA4 antibody (STJ11105691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-PDIA4 antibody (200-300) (STJ11105691)

SKU:
STJ11105691

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein Disulfide-Isomerase A4 (200-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDIA4
Gene ID: 9601
Uniprot ID: PDIA4_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PDIA4 (NP_004902.1).
Immunogen Sequence: EFYAPWCGHCKKLAPEYEKA AKELSKRSPPIPLAKVDATA ETDLAKRFDVSGYPTLKIFR KGRPYDYNGPREKYGIVDYM IEQSGPPSKEILTLKQVQEF L
Protein Name Protein Disulfide-Isomerase A4
Endoplasmic Reticulum Resident Protein 70
Er Protein 70
Erp70
Endoplasmic Reticulum Resident Protein 72
Er Protein 72
Erp-72
Erp72
Cellular Localisation Endoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Protein Disulfide-Isomerase A4 antibody
Anti-Endoplasmic Reticulum Resident Protein 70 antibody
Anti-Er Protein 70 antibody
Anti-Erp70 antibody
Anti-Endoplasmic Reticulum Resident Protein 72 antibody
Anti-Er Protein 72 antibody
Anti-Erp-72 antibody
Anti-Erp72 antibody
Anti-PDIA4 antibody
Anti-ERP70 antibody
Anti-ERP72 antibody

Information sourced from Uniprot.org

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