Anti-PDIA3 antibody (111-160 aa) (STJ96585)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human PDIA3 at the amino acid range 111-160
General Information
| Short Description | Rabbit polyclonal anti-Protein disulfide-isomerase A3 (111-160 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PDIA3 |
| Gene ID | 2923 |
| Uniprot ID | PDIA3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human PDIA3 at the amino acid range 111-160 |
| Immunogen Region | 111-160 aa |
| Specificity | ERp57 Polyclonal Antibody detects endogenous levels of ERp57 protein. |
Additional Info
| Post Translational Modifications | Within the major histocompatibility complex class I (MHC I) peptide loading complex forms reversible disulfide-linked heterodimers with TAPBP as part of its protein folding chaperone activity. This is essential to assist the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Phosphorylated. |
| Function | Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity. |
| Protein Name | Protein Disulfide-Isomerase A358 Kda Glucose-Regulated Protein58 Kda Microsomal ProteinP58Disulfide Isomerase Er-60Endoplasmic Reticulum Resident Protein 57Er Protein 57Erp57Endoplasmic Reticulum Resident Protein 60Er Protein 60Erp60 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-901042Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic ReticulumEndoplasmic Reticulum LumenMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Protein Disulfide-Isomerase A3 antibodyAnti-58 Kda Glucose-Regulated Protein antibodyAnti-58 Kda Microsomal Protein antibodyAnti-P58 antibodyAnti-Disulfide Isomerase Er-60 antibodyAnti-Endoplasmic Reticulum Resident Protein 57 antibodyAnti-Er Protein 57 antibodyAnti-Erp57 antibodyAnti-Endoplasmic Reticulum Resident Protein 60 antibodyAnti-Er Protein 60 antibodyAnti-Erp60 antibodyAnti-PDIA3 antibodyAnti-ERP57 antibodyAnti-ERP60 antibodyAnti-GRP58 antibody |
Information sourced from Uniprot.org