• Immunofluorescence analysis of NIH/3T3 cells using PDIA2 Rabbit polyclonal antibody (STJ114937) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PDIA2 Rabbit polyclonal antibody (STJ114937) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PDIA2 antibody (326-525) (STJ114937)

SKU:
STJ114937

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDIA2 (326-525) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDIA2
Gene ID: 64714
Uniprot ID: PDIA2_HUMAN
Immunogen Region: 326-525
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 326-525 of human PDIA2 (NP_006840.2).
Immunogen Sequence: FGLKAEAAPTLRLVNLETTK KYAPVDGGPVTAASITAFCH AVLNGQVKPYLLSQEIPPDW DQRPVKTLVGKNFEQVAFDE TKNVFVKFYAPWCTHCKEMA PAWEALAEKYQDHEDIIIAE LDATANELDAFAVHGFPTLK YFPAGPGRKVIEYKSTRDLE TFSKFLDNGGVLPTEEPPEE PAAPFPEPPANSTMGSKEEL
Tissue Specificity Highly expressed in pancreas (at protein level).
Post Translational Modifications The disulfide-linked homodimer exhibits an enhanced chaperone activity. Glycosylated.
Function Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Protein Name Protein Disulfide-Isomerase A2
Pancreas-Specific Protein Disulfide Isomerase
Pdip
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Protein Disulfide-Isomerase A2 antibody
Anti-Pancreas-Specific Protein Disulfide Isomerase antibody
Anti-Pdip antibody
Anti-PDIA2 antibody
Anti-PDIP antibody

Information sourced from Uniprot.org

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