ELISA Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AIF1/IBA1 antibody (1-100) [S7MR] (STJ11103127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-AIF1/IBA1 antibody (1-147 aa) [SRM] (STJ11106865)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-AIF1/IBA1 antibody (1-147 aa) [SRM] (STJ11106866)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-AIF1/IBA1 isoform 1 and 3 antibody (C-Term) (STJ73855)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$261.23

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Anti-AIF1/IBA1 isoform 1 and 3 antibody (C-Term) (STJ73856)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

$261.23

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Anti-AIF1/IBA1 isoform 1 and 3 antibody (C-Term) (STJ73857)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Rat

$261.23

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Biotinylated STJ70166 (0. 1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-AIF1/IBA1, biotinylated-Isoform 1/3 antibody (C-Term) {Biotin} (STJ73271)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Pig

$421.88

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STJ70166 (0. 3µg/ml) staining of Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIF1/IBA1-Isoform 1/3 antibody (C-Term) (STJ70166)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Pig

$421.88

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STJ70928 (4µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Anti-AIF1/IBA1-Isoform 1/3 antibody (C-Term) (STJ70928)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human

$421.88

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STJ70888 Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-AIF1/IBA1-Isoform 3 antibody (Internal) (STJ70888)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Mouse/Dog

$421.88

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STJ73111 (0. 1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIFM1 antibody (183-195) (STJ73111)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC

Reactivity :

Human/Cow

$421.88

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Immunofluorescence analysis of C6 cells using AIF antibody (STJ11103814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AIFM1 antibody (334-613) (STJ11103814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Confocal immunofluorescence analysis of HeLa cells using [KO Validated] AIF Rabbit polyclonal antibody (STJ22557) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-AIFM1 antibody (334-613) (STJ22557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human oophoroma using AIF antibody (STJ11101683) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AIFM1 antibody (450-550) [S3MR] (STJ11101683)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various cells using AIF-M1 Polyclonal Antibody diluted at 1：1000

Anti-AIFM1 antibody (51-100 aa) (STJ91514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ73128 (0. 01µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIFM1 antibody (C-Term) (STJ73128)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$421.88

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Western blot analysis using AIF-M1 monoclonal antibody against NIH/3T3 (1) , Jurkat (2) , HeLa (3) , HepG2 (4) , MOLT4 (5) , C6 (6) , RAJI (7) , Cos7 (8) and PC-12 (9) cell lysate.

Anti-AIFM1 antibody [4E7] (STJ97819)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$194.40 - $469.80

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Western blot analysis of various cells using AMID Polyclonal Antibody

Anti-AIFM2 antibody (141-190 aa) (STJ91575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

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Western blot analysis of extracts of HepG2 cells, using AIFM2/FSP1 antibody (STJ114022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-AIFM2 antibody (43-371) (STJ114022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using AIFM3 Rabbit pAb (STJ111329) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-AIFM3 antibody (1-200) (STJ111329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using AIFL Polyclonal Antibody

Anti-AIFM3 antibody (10-59 aa) (STJ91513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-AIFM3 antibody (Internal) (STJ72111)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human/Mouse/Rat/Dog/Cow

$261.23

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Western blot analysis of various cells using AIG-1 Polyclonal Antibody

Anti-AIG1 antibody (196-245 aa) (STJ91515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of A-431 cells using AIM2 Rabbit polyclonal antibody (STJ22558) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AIM2 antibody (130-337) (STJ22558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from SP2/0 cells using AIM2 Rabbit polyclonal antibody (STJ11105625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AIM2 antibody (158-354) (STJ11105625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Western blot with Anti-AIM2 Antibody (STJ500057) of mouse cortex at 1:500 dilution in buffer.

Anti-AIM2 antibody (240-340) (STJ500057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

$577.13

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Anti-AIM2 antibody (240-340) {Biotin} (STJ500058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

$713.48

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Western blot with Anti-AIM2 Antibody (FITC) (STJ500059) of cerebral cortex at 1:500 dilution in buffer.

Anti-AIM2 antibody (240-340) {FITC} (STJ500059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

$713.48

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Western blot analysis of Rat spleen, using Aim2 antibody (STJ11104964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Aim2 antibody (50-150) (STJ11104964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$151.88 - $708.08

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Immunofluorescence analysis of Hela cell. 1, AIM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-AIM2 antibody (51-100 aa) (STJ91516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ72169 (1µg/ml) staining of IFNbeta-treated WI-38 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIM2 antibody (C-Term) (STJ72169)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

$421.88

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STJ72168 (0. 3µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIM2 antibody (Internal) (STJ72168)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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Anti-Aim2 antibody (Internal) (STJ72928)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Mouse/Rat

$261.23

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Immunohistochemistry analysis of paraffin-embedded mouse heart using AIMP1 antibody (STJ27254) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AIMP1 antibody (25-180) (STJ27254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of KB cells using EMAP II Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-AIMP1 antibody (91-140 aa) (STJ97338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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STJ72797 (0. 1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIMP1/SCYE1 antibody (137-149) (STJ72797)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Dog/Pig

$421.88

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STJ72796 (0. 3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIMP1/SCYE1 antibody (N-Term) (STJ72796)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Pig/Cow

$421.88

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Biotinylated STJ72797 (0. 1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIMP1/SCYE1, Biotinylated antibody (137-149) {Biotin} (STJ73510)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog/Pig

$421.88

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Anti-AIMP2 antibody (141-320 aa) (STJ11107491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-AIMP2 antibody (STJA0004417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$189.68 - $560.93

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Western blot analysis of lysates from wild type (WT) and AIP knockout (KO) HeLa cells, using [KO Validated] AIP Rabbit polyclonal antibody (STJ11100845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AIP antibody (1-330) (STJ11100845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using AIP antibody (STJ114420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-AIP antibody (1-330) (STJ114420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ71174 (0. 02µg/ml) staining of NIH/3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIP/ARA9 antibody (C-Term) (STJ71174)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

$421.88

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Anti-AIP1/MAGI2 antibody (C-Term) (STJ70473)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human

$261.23

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Immunofluorescence analysis of Y79 cells using AIPL1 antibody (STJ116048) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIPL1 antibody (1-384) (STJ116048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of Y79 cells using AIPL1 antibody (STJ28541) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIPL1 antibody (1-384) (STJ28541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various lysates using AIRE Rabbit pAb (STJ116116) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-AIRE antibody (1-280) (STJ116116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from HeLa cells, treated with Hu 2nM 24h, using AIRE Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-AIRE antibody (126-175 aa) (STJ91519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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