| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-AGK (1-422) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S9MM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AGK |
| Gene ID: |
55750 |
| Uniprot ID: |
AGK_HUMAN |
| Immunogen Region: |
1-422 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-422 of human AGK (NP_060708.1). |
| Immunogen Sequence: |
MTVFFKTLRNHWKKTTAGLC LLTWGGHWLYGKHCDNLLRR AACQEAQVFGNQLIPPNAQV KKATVFLNPAACKGKARTLF EKNAAPILHLSGMDVTIVKT DYEGQAKKLLELMENTDVII VAGGDGTLQEVVTGVLRRTD EATFSKIPIGFIPLGETSSL SHTLFAESGNKVQHITDATL AIVKGETVPLDVLQIKGEKE QPVFAMTGLRWGSFRDAGVK VSKYWYLGPLKIKAAHFFS |
| Tissue Specificity | Highly expressed in muscle, heart, kidney and brain. |
| Function | Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Phosphorylates ceramide. Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol. Independently of its lipid kinase activity, acts as a component of the TIM22 complex. The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force. In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. |
| Protein Name | Acylglycerol Kinase - MitochondrialHagk Multiple Substrate Lipid KinaseHsmulkMulkMulti-Substrate Lipid Kinase |
| Database Links | Reactome: R-HSA-1483206Reactome: R-HSA-6802952 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMitochondrion Intermembrane SpaceLocalizes In The Mitochondrion Intermembrane SpaceWhere It Associates With The Inner MembraneIt Is Unclear Whether The N-Terminal Hydrophobic Region Forms A Transmembrane Region Or Associates With The Membrane Without Crossing It |
| Alternative Antibody Names | Anti-Acylglycerol Kinase - Mitochondrial antibodyAnti-Hagk antibodyAnti-Multiple Substrate Lipid Kinase antibodyAnti-Hsmulk antibodyAnti-Mulk antibodyAnti-Multi-Substrate Lipid Kinase antibodyAnti-AGK antibodyAnti-MULK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
