Mouse MMP-2 (72 Kda Type Iv Collagenase) Antibody Pair (STJAP00289)
General Information
| Short Description | This MMP-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Mmp2 |
| Gene ID | 17390 |
| Uniprot ID | MMP2_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse MMP-2 protein.Detection Immunogen: Recombinant Mouse MMP-2 protein. |
Additional Info
| Function | Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. |
| Protein Name | 72 Kda Type Iv Collagenase72 Kda GelatinaseGelatinase AMatrix Metalloproteinase-2Mmp-2 Cleaved Into - Pex |
| Database Links | Reactome: R-MMU-1442490Reactome: -MMU-1474228Reactome: -MMU-1592389Reactome: -MMU-3928665Reactome: -MMU-9009391 |
| Cellular Localisation | Isoform 1: SecretedExtracellular SpaceExtracellular MatrixMembraneNucleusColocalizes With Integrin Alphav/Beta3 At The Membrane Surface In Angiogenic Blood Vessels And MelanomasFound In MitochondriaAlong MicrofibrilsAnd In Nuclei Of CardiomyocytesIsoform 2: CytoplasmMitochondrion |
| Alternative Antibody Pair Names | 72 Kda Type Iv Collagenase antibody pair72 Kda Gelatinase antibody pairGelatinase A antibody pairMatrix Metalloproteinase-2 antibody pairMmp-2 Cleaved Into - Pex antibody pairMmp2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse MMP-2 (72 Kda Type Iv Collagenase) Antibody Pair (STJAP00289)
This antibody pair is designed to detect mouse MMP-2 (72 kDa Type IV Collagenase), corresponding to UniProt accession P33434. Reactivity is confirmed for mouse only.
Both antibodies are polyclonal, raised in goat, and supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotin-conjugated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both antibodies were raised against recombinant mouse MMP-2 protein. Both have also been purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.