Mouse IL-16 (Pro-Interleukin-16 Cleaved Into-Interleukin-16) Antibody Pair (STJAP00548)
General Information
| Short Description | This IL-16 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Il16 |
| Gene ID | 16170 |
| Uniprot ID | IL16_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse IL-16 protien.Detection Immunogen: Recombinant Mouse IL-16 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Synthesized as a chemo-attractant inactive precursor which is proteolytically cleaved by caspase-3 to yield IL-16. |
| Function | Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane. Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. |
| Protein Name | Pro-Interleukin-16 Cleaved Into - Interleukin-16Il-16Lymphocyte Chemoattractant FactorLcf |
| Database Links | Reactome: R-MMU-449836 |
| Cellular Localisation | SecretedIsoform 1: CytoplasmColocalizes With Grin2c In Neuronal Cell Bodies And NeuritesIsoform 2: CytoplasmNucleus |
| Alternative Antibody Pair Names | Pro-Interleukin-16 Cleaved Into - Interleukin-16 antibody pairIl-16 antibody pairLymphocyte Chemoattractant Factor antibody pairLcf antibody pairIl16 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IL-16 (Pro-Interleukin-16 Cleaved Into-Interleukin-16) Antibody Pair (STJAP00548)
This antibody pair detects mouse IL-16 (Pro-Interleukin-16 cleaved into Interleukin-16), corresponding to UniProt accession O54824. Reactivity is confirmed for mouse samples only.
Both antibodies are rabbit-raised and supplied at 0.5 mg/mL. The capture antibody is unconjugated, formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is biotinylated and formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This pair is validated for use in ELISA. It is suitable for detecting IL-16 in serum, plasma, and other biological fluids.
Both the capture and detection antibodies were raised against recombinant mouse IL-16 protein. No further detail on immunogen region is provided in the product specifications.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Both antibodies were purified using Protein A or Protein G affinity chromatography.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.