| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This mouse ANXA2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Anxa2 |
| Gene ID: |
12306 |
| Uniprot ID: |
ANXA2_MOUSE |
| Specificity: |
This kit recognizes Mouse ANXA2 in samples. No significant cross-reactivity or interference between Mouse ANXA2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | ISGylated. |
| Function | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. |
| Protein Name | Annexin A2Annexin IiAnnexin-2Calpactin I Heavy ChainCalpactin-1 Heavy ChainChromobindin-8Lipocortin IiPlacental Anticoagulant Protein IvPap-IvProtein IP36 |
| Database Links | Reactome: R-MMU-6798695Reactome: -MMU-75205 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneMelanosomeIn The Lamina Beneath The Plasma Membrane |
| Alternative CLIA Names | Annexin A2 CLIA kitAnnexin Ii CLIA kitAnnexin-2 CLIA kitCalpactin I Heavy Chain CLIA kitCalpactin-1 Heavy Chain CLIA kitChromobindin-8 CLIA kitLipocortin Ii CLIA kitPlacental Anticoagulant Protein Iv CLIA kitPap-Iv CLIA kitProtein I CLIA kitP36 CLIA kitAnxa2 CLIA kitAnx2 CLIA kitCal1h CLIA kit |
| Specificity | This kit recognizes Mouse ANXA2 in samples. No significant cross-reactivity or interference between Mouse ANXA2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-115 | 107 |
| EDTA plasma(n=8) | 100-115 | 106 |
| Cell culture media(n=8) | 101-118 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.00 | 2.65 | 7.15 | 0.91 | 2.42 | 7.84 |
| Standard deviation | 0.09 | 0.22 | 0.57 | 0.11 | 0.17 | 0.70 |
| CV (%) | 9.00 | 8.30 | 7.97 | 12.09 | 7.02 | 8.93 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
