Mouse Alpha-synuclein (Alpha-synuclein) Antibody Pair (STJAP00757)
General Information
| Short Description | This Alpha-synuclein antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R59-4X-6/R83-8F-8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Snca |
| Gene ID | 20617 |
| Uniprot ID | SYUA_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in brain (at protein level). Expressed in the striatum (at protein level). Highly expressed in presynaptic terminals in the central nervous system. |
| Post Translational Modifications | Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
| Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
| Protein Name | Alpha-SynucleinNon-A Beta Component Of Ad AmyloidNon-A4 Component Of Amyloid PrecursorNacp |
| Database Links | Reactome: R-MMU-9833482 |
| Cellular Localisation | CytoplasmMembraneNucleusSynapseSecretedCell ProjectionAxonMembrane-Bound In Dopaminergic NeuronsExpressed And Colocalized With Septin4 In Dopaminergic Axon TerminalsEspecially At The Varicosities |
| Alternative Antibody Pair Names | Alpha-Synuclein antibody pairNon-A Beta Component Of Ad Amyloid antibody pairNon-A4 Component Of Amyloid Precursor antibody pairNacp antibody pairSnca antibody pairSyn antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse Alpha-synuclein (Alpha-synuclein) Antibody Pair (STJAP00757)
This antibody pair is designed to detect mouse Alpha-synuclein (UniProt O55042) and has confirmed reactivity against the mouse target only. Researchers working with human or rat samples should verify cross-reactivity independently before use.
Both antibodies are supplied as unconjugated, affinity-purified rabbit monoclonal IgGs (clones R59-4X-6 and R83-8F-8) at a concentration of 1 mg/mL, formulated in 0.01M PBS pH 7.2. The user is responsible for determining the appropriate working dilution for each component.
This pair has been validated for use in ELISA. Suitability for other assay formats has not been confirmed based on the available product data.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, avoiding repeated freeze/thaw cycles to maintain activity.
Yes, both the capture and detection antibodies are rabbit-derived monoclonal IgGs, raised against a recombinant protein immunogen. Using two antibodies from the same host may require consideration when selecting secondary detection reagents in sandwich ELISA formats.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.