| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
IP3 receptor Blocking Peptide is synthetically produced from the 100-150 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
ITPR1 |
| Gene ID: |
3708 |
| Uniprot ID: |
ITPR1_HUMAN |
| Immunogen Region: |
100-150 |
| Immunogen: |
Synthetic peptide taken within amino acid region 100-150 on rat Inosital 1, 4, 5-triphosphate receptor type 2 protein. |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Polyubiquitinated. Polyubiquitination targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked. Phosphorylated by cAMP kinase (PKA) enhances calcium release. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1. Phosphorylated on tyrosine residues. Palmitoylated by ZDHHC6 in immune cells, leading to regulation of ITPR1 stability and function. |
| Function | Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix. Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations. |
| Peptide Name | Inositol 1 -4 -5-Trisphosphate-Gated Calcium Channel Itpr1Ip3 Receptor Isoform 1Ip3r 1Insp3r1Inositol 1 -4 -5 Trisphosphate ReceptorInositol 1 -4 -5-Trisphosphate Receptor Type 1Type 1 Inositol 1 -4 -5-Trisphosphate ReceptorType 1 Insp3 Receptor |
| Database Links | Reactome: R-HSA-112043Reactome: R-HSA-114508Reactome: R-HSA-139853Reactome: R-HSA-1489509Reactome: R-HSA-2029485Reactome: R-HSA-2871809Reactome: R-HSA-381676Reactome: R-HSA-4086398Reactome: R-HSA-418457Reactome: R-HSA-422356Reactome: R-HSA-5218921Reactome: R-HSA-5578775Reactome: R-HSA-5607763Reactome: R-HSA-9664323Reactome: R-HSA-983695 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCytoplasmic VesicleSecretory Vesicle MembraneCytoplasmPerinuclear RegionFound In A Complex With Hspa9 And Vdac1 At The Endoplasmic Reticulum-Mitochondria Contact Sites |
| Alternative Peptide Names | Inositol 1 -4 -5-Trisphosphate-Gated Calcium Channel Itpr1 proteinIp3 Receptor Isoform 1 proteinIp3r 1 proteinInsp3r1 proteinInositol 1 -4 -5 Trisphosphate Receptor proteinInositol 1 -4 -5-Trisphosphate Receptor Type 1 proteinType 1 Inositol 1 -4 -5-Trisphosphate Receptor proteinType 1 Insp3 Receptor proteinITPR1 proteinINSP3R1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
