IP3 receptor Blocking Peptide is synthetically produced from the 100-150 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region 100-150 on rat Inosital 1, 4, 5-triphosphate receptor type 2 protein.
Immunogen Region
100-150
Additional Info
Tissue Specificity
Widely expressed.
Post Translational Modifications
Polyubiquitinated. Polyubiquitination targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked. Phosphorylated by cAMP kinase (PKA) enhances calcium release. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1. Phosphorylated on tyrosine residues. Palmitoylated by ZDHHC6 in immune cells, leading to regulation of ITPR1 stability and function.
Function
Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix. Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations.
Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCytoplasmic VesicleSecretory Vesicle MembraneCytoplasmPerinuclear RegionFound In A Complex With Hspa9 And Vdac1 At The Endoplasmic Reticulum-Mitochondria Contact Sites
