Human Vitronectin/V75/VTN protein (Recombinant) (C-His) (STJP001377)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Vitronectin/V75/VTN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp20-Leu478) of human Vitronectin/VTN (Accession #NP_000629.3) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Vitronectin/V75/VTN-C-His protein was developed in hek293 cells using the region Asp20-Leu478. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. When 5×104 cells/well are added to Vitronectin coated plates (5 µg/mL with 100 µL/well) , approximately >85% will adhere after 30 mi |
| Determination Method | <1 EU/Mu g |
Target Information
| Gene Symbol | VTN |
| Gene ID | 7448 |
| Uniprot ID | VTNC_HUMAN |
| Immunogen | Recombinant Human Vitronectin/V75/VTN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp20-Leu478) of human Vitronectin/VTN (Accession #NP_000629.3) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Asp20-Leu478 |
| Immunogen Sequence | DQESCKGRCTEGFNVDKKCQ CDELCSYYQSCCTDYTAECK PQVTRGDVFTMPEDEYTVYD DGEEKNNATVHEQVGGPSLT SDLQAQSKGNPEQTPVLKPE EEAPAPEVGASKPEGIDSRP ETLHPGRPQPPAEEELCSGK PFDAFTDLKNGSLFAFRGQY CYELDEKAVRPGYPKLIRDV WGIEGPIDAAFTRINCQGKT YLFKGSQYWRFEDGVLDPDY PRNISDGFDGIPDNVDAAL |
Additional Info
| Post Translational Modifications | Sulfated on tyrosine residues. N- and O-glycosylated. Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. Phosphorylation sites are present in the extracellular medium. |
| Function | Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. |
| Protein Name | VitronectinVnS-ProteinSerum-Spreading FactorV75 Cleaved Into - Vitronectin V65 Subunit - Vitronectin V10 Subunit - Somatomedin-B |
| Database Links | Reactome: R-HSA-2129379Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-3000178Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedExtracellular SpaceParasitophorous Vacuole(Microbial Infection) In PFalciparum-Infected Red Blood CellsVtn Internalization Is Detected At The Early Trophozoite StageColocalizes With Sera5 At The Schizont Stage And With Sera5 P47 At The Merozoite Surface |
| Alternative Protein Names | Vitronectin proteinVn proteinS-Protein proteinSerum-Spreading Factor proteinV75 Cleaved Into - Vitronectin V65 Subunit - Vitronectin V10 Subunit - Somatomedin-B proteinVTN protein |
Information sourced from Uniprot.org