| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Vitronectin/V75/VTN-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Immunoreactivity: |
Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. When 5×104 cells/well are added to Vitronectin coated plates (5 µg/mL with 100 µL/well) , approximately >85% will adhere after 30 minutes at 37 |
| Gene Symbol: |
VTN |
| Gene ID: |
7448 |
| Uniprot ID: |
VTNC_HUMAN |
| Immunogen: |
Recombinant Human Vitronectin/V75/VTN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp20-Leu478) of human Vitronectin/VTN (Accession #NP_000629.3) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | Sulfated on tyrosine residues. N- and O-glycosylated. Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. Phosphorylation sites are present in the extracellular medium. |
| Function | Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. |
| Protein Name | VitronectinVnS-ProteinSerum-Spreading FactorV75 Cleaved Into - Vitronectin V65 Subunit - Vitronectin V10 Subunit - Somatomedin-B |
| Database Links | Reactome: R-HSA-2129379Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-3000178Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedExtracellular SpaceParasitophorous Vacuole(Microbial Infection) In PFalciparum-Infected Red Blood CellsVtn Internalization Is Detected At The Early Trophozoite StageColocalizes With Sera5 At The Schizont Stage And With Sera5 P47 At The Merozoite Surface |
| Alternative Protein Names | Vitronectin proteinVn proteinS-Protein proteinSerum-Spreading Factor proteinV75 Cleaved Into - Vitronectin V65 Subunit - Vitronectin V10 Subunit - Somatomedin-B proteinVTN protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
