Human uPA/PLAU protein (Recombinant) (C-His) (STJP000921)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human uPA/PLAU Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Leu431) of human Urokinase/PLAU (Accession #NP_002649.1) fused with a 8×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human uPA/PLAU-C-His protein was developed in hek293 cells using the region Met1-Leu431. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Supplied as a 0.22 Mu m filtered solution in 20mM HEPES, 150mM NaCl, 2mM CaCl, 10% Glycerol, pH 7.5 |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human uPAR Protein at 1 Mu g/mL (100 Mu L/well) can bind PLAU with a linear range of 0.031-1.014 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | PLAU |
| Gene ID | 5328 |
| Uniprot ID | UROK_HUMAN |
| Immunogen | Recombinant Human uPA/PLAU Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Leu431) of human Urokinase/PLAU (Accession #NP_002649.1) fused with a 8×His tag at the C-terminus. |
| Immunogen Region | Met1-Leu431 |
| Immunogen Sequence | MRALLARLLLCVLVVSDSKG SNELHQVPSNCDCLNGGTCV SNKYFSNIHWCNCPKKFGGQ HCEIDKSKTCYEGNGHFYRG KASTDTMGRPCLPWNSATVL QQTYHAHRSDALQLGLGKHN YCRNPDNRRRPWCYVQVGLK PLVQECMVHDCADGKKPSSP PEELKFQCGQKTLRPRFKII GGEFTTIENQPWFAAIYRRH RGGSVTYVCGGSLISPCWVI SATHCFIDYPKKEDYIVYL |
Additional Info
| Tissue Specificity | Expressed in the prostate gland and prostate cancers. |
| Post Translational Modifications | Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4. |
| Function | Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. |
| Protein Name | Urokinase-Type Plasminogen ActivatorU-Plasminogen ActivatorUpa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-75205 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Urokinase-Type Plasminogen Activator proteinU-Plasminogen Activator proteinUpa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B proteinPLAU protein |
Information sourced from Uniprot.org