Human TSPAN30/CD63 (Cd63 Antigen) Antibody Pair (STJAP00470)
General Information
| Short Description | This TSPAN30/CD63 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | CD63 |
| Gene ID | 967 |
| Uniprot ID | CD63_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human TSPAN30/CD63 protien.Detection Immunogen: Recombinant Human TSPAN30/CD63 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). |
| Function | Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. |
| Protein Name | Cd63 AntigenGranulophysinLysosomal-Associated Membrane Protein 3Lamp-3Lysosome Integral Membrane Protein 1Limp1Melanoma-Associated Antigen Me491Oma81hOcular Melanoma-Associated AntigenTetraspanin-30Tspan-30Cd Antigen Cd63 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLysosome MembraneLate Endosome MembraneEndosomeMultivesicular BodyMelanosomeSecretedExtracellular ExosomeCell SurfaceAlso Found In Weibel-Palade Bodies Of Endothelial CellsLocated In Platelet Dense GranulesDetected In A Subset Of Pre-MelanosomesDetected On Intralumenal Vesicles (Ilvs) Within Multivesicular Bodies |
| Alternative Antibody Pair Names | Cd63 Antigen antibody pairGranulophysin antibody pairLysosomal-Associated Membrane Protein 3 antibody pairLamp-3 antibody pairLysosome Integral Membrane Protein 1 antibody pairLimp1 antibody pairMelanoma-Associated Antigen Me491 antibody pairOma81h antibody pairOcular Melanoma-Associated Antigen antibody pairTetraspanin-30 antibody pairTspan-30 antibody pairCd Antigen Cd63 antibody pairCD63 antibody pairMLA1 antibody pairTSPAN30 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human TSPAN30/CD63 (Cd63 Antigen) Antibody Pair (STJAP00470)
This antibody pair is designed to detect human TSPAN30/CD63 (UniProt P08962) and has confirmed reactivity with human samples only. No cross-reactivity with other species is documented in the provided product details.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated; both are raised in rabbit and provided at 0.5 mg/mL. The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol (pH 7.5), while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol (pH 7.5).
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human TSPAN30/CD63 protein. Both antibodies have also been purified by antigen affinity chromatography.
This antibody pair is suitable for use with serum, plasma, and other biological fluids. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both antibodies should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.