Human Serpin A1/Alpha-1-antitrypsin protein (Recombinant) (C-His) (STJP002614)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Serpin A1/Alpha-1-antitrypsin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu25-Lys418) of human alpha 1 Antitrypsin (Accession #NP_000286.3) fused with a 6×His tag at the C-
General Information
| Short Description | Recombinant-Human Serpin A1/Alpha-1-antitrypsin-C-His protein was developed in hek293 cells using the region Glu25-Lys418. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is approximately <0.4 nM. |
| Determination Method | < 1.0 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | SERPINA1 |
| Gene ID | 5265 |
| Uniprot ID | A1AT_HUMAN |
| Immunogen | Recombinant Human Serpin A1/Alpha-1-antitrypsin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu25-Lys418) of human alpha 1 Antitrypsin (Accession #NP_000286.3) fused with a 6×His tag at the C- |
| Immunogen Region | Glu25-Lys418 |
| Immunogen Sequence | EDPQGDAAQKTDTSHHDQDH PTFNKITPNLAEFAFSLYRQ LAHQSNSTNIFFSPVSIATA FAMLSLGTKADTHDEILEGL NFNLTEIPEAQIHEGFQELL RTLNQPDSQLQLTTGNGLFL SEGLKLVDKFLEDVKKLYHS EAFTVNFGDTEEAKKQINDY VEKGTQGKIVDLVKELDRDT VFALVNYIFFKGKWERPFEV KDTEEEDFHVDQVTTVKVPM MKRLGMFNIQHCKKLSSWV |
Additional Info
| Post Translational Modifications | N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases. |
| Function | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). |
| Protein Name | Alpha-1-AntitrypsinAlpha-1 Protease InhibitorAlpha-1-AntiproteinaseSerpin A1 Cleaved Into - Short Peptide From AatSpaat |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-204005Reactome: R-HSA-381426Reactome: R-HSA-5694530Reactome: R-HSA-6798695Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedEndoplasmic ReticulumThe S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic ReticulumShort Peptide From Aat: SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Alpha-1-Antitrypsin proteinAlpha-1 Protease Inhibitor proteinAlpha-1-Antiproteinase proteinSerpin A1 Cleaved Into - Short Peptide From Aat proteinSpaat proteinSERPINA1 proteinAAT proteinPI proteinPRO0684 proteinPRO2209 protein |
Information sourced from Uniprot.org