Human S100A12 (Protein S100-A12) Antibody Pair (STJAP00499)
General Information
| Short Description | This S100A12 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | S100A12 |
| Gene ID | 6283 |
| Uniprot ID | S10AC_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human S100A12 protien.Detection Immunogen: Recombinant Human S100A12 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases.MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. |
| Protein Name | Protein S100-A12CgrpCalcium-Binding Protein In Amniotic Fluid 1Caaf1Calgranulin-CCagcExtracellular Newly Identified Rage-Binding ProteinEn-RageMigration Inhibitory Factor-Related Protein 6Mrp-6P6Neutrophil S100 ProteinS100 Calcium-Binding Protein A12 Cleaved Into - Calcitermin |
| Database Links | Reactome: R-HSA-445989Reactome: R-HSA-6798695Reactome: R-HSA-879415Reactome: R-HSA-933542 |
| Cellular Localisation | SecretedCytoplasmCytoskeletonCell MembranePeripheral Membrane ProteinPredominantly Localized In The CytoplasmUpon Elevation Of The Intracellular Calcium LevelTranslocated From The Cytoplasm To The Cytoskeleton And The Cell MembraneUpon Neutrophil Activation Is Secreted Via A Microtubule-MediatedAlternative Pathway |
| Alternative Antibody Pair Names | Protein S100-A12 antibody pairCgrp antibody pairCalcium-Binding Protein In Amniotic Fluid 1 antibody pairCaaf1 antibody pairCalgranulin-C antibody pairCagc antibody pairExtracellular Newly Identified Rage-Binding Protein antibody pairEn-Rage antibody pairMigration Inhibitory Factor-Related Protein 6 antibody pairMrp-6 antibody pairP6 antibody pairNeutrophil S100 Protein antibody pairS100 Calcium-Binding Protein A12 Cleaved Into - Calcitermin antibody pairS100A12 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human S100A12 (Protein S100-A12) Antibody Pair (STJAP00499)
This antibody pair detects human S100A12 (Protein S100-A12, UniProt P80511) and is validated for reactivity against human samples only. No cross-reactivity with other species has been documented in the provided product data.
Both antibodies are rabbit-raised and purified by antigen affinity. The capture antibody is unconjugated and the detection antibody is biotinylated, and both are supplied at 0.5 mg/mL.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product documentation.
This antibody pair has been tested with serum, plasma, and other biological fluids. Suitability with additional sample types is not specified in the product data.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Both the capture and detection antibodies were raised against recombinant human S100A12 protein. Both antibodies were subsequently purified by antigen affinity chromatography.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.