Human S100A1 (S100A1) Antibody Pair (STJAP00817)
General Information
| Short Description | This S100A1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R39-3P-7/R68-6V-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | S100A1 |
| Gene ID | 6271 |
| Uniprot ID | S10A1_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. |
| Post Translational Modifications | Glutathionylated.glutathionylation increases affinity to calcium about 10-fold. |
| Function | Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole. |
| Protein Name | Protein S100-A1S-100 Protein Alpha ChainS-100 Protein Subunit AlphaS100 Calcium-Binding Protein A1 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-166058Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5686938 |
| Cellular Localisation | CytoplasmSarcoplasmic ReticulumMitochondrion |
| Alternative Antibody Pair Names | Protein S100-A1 antibody pairS-100 Protein Alpha Chain antibody pairS-100 Protein Subunit Alpha antibody pairS100 Calcium-Binding Protein A1 antibody pairS100A1 antibody pairS100A antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human S100A1 (S100A1) Antibody Pair (STJAP00817)
This antibody pair is designed to detect human S100A1 (UniProt P23297) and has confirmed reactivity against the human protein only. Researchers working with non-human samples should validate cross-reactivity independently before use.
This antibody pair is validated for use in ELISA. It is intended for sandwich ELISA development, with one antibody serving as capture and the other as detection.
Both antibodies are rabbit monoclonal IgGs (clone IDs R39-3P-7 and R68-6V-1), supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2, and purified by affinity chromatography. Detection antibody conjugation to a reporter, such as biotin or HRP, would need to be performed or sourced separately by the end user.
Optimal working concentrations for both the capture and detection antibodies are to be determined by the end user. Titration experiments are recommended to establish the appropriate dilutions for each specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquoting and storing at -20°C is recommended, and repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Both antibodies were raised in rabbit against a recombinant human S100A1 protein. No specific cross-reactivity or exclusivity data against related S100 family members is provided in the current product details, so users should conduct their own specificity testing if required for their experimental context.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.