Human QTRT1 protein (Recombinant) (His-Tag) (STJP019173)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human QTRT1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) containing 30% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | QTRT1 |
| Gene ID | 81890 |
| Uniprot ID | TGT_HUMAN |
| Accession Number | NP_112486 |
| Immunogen | Human |
| Immunogen Region | 1-403aa |
| Immunogen Sequence |
Additional Info
| Function | Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product. Modification of cytoplasmic tRNAs with queuosine controls the elongation speed of cognate codons, thereby ensuring the correct folding of nascent proteins to maintain proteome integrity. |
| Protein Name | Queuine Trna-Ribosyltransferase Catalytic Subunit 1Guanine Insertion EnzymeTrna-Guanine Transglycosylase |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | CytoplasmMitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmic SideWeakly Associates With MitochondriaPossibly Via Qtrt2 |
| Alternative Protein Names | Queuine Trna-Ribosyltransferase Catalytic Subunit 1 proteinGuanine Insertion Enzyme proteinTrna-Guanine Transglycosylase proteinQTRT1 proteinTGT proteinTGUT protein |
Information sourced from Uniprot.org