Human PNPT1 protein (Recombinant) (His-Tag) (STJP019111)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human PNPT1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PNPT1 |
| Gene ID | 87178 |
| Uniprot ID | PNPT1_HUMAN |
| Accession Number | NP_149100.2 |
| Immunogen | Human |
| Immunogen Region | 46-783aa |
| Immunogen Sequence |
Additional Info
| Function | RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration.regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity.degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells.specifically and selectively degrades miR-221, preferentially. Also plays a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. |
| Protein Name | Polyribonucleotide Nucleotidyltransferase 1 - Mitochondrial3'-5' Rna Exonuclease Old35Pnpase Old-35Polynucleotide Phosphorylase 1Pnpase 1Polynucleotide Phosphorylase-Like Protein |
| Database Links | Reactome: R-HSA-9836573 |
| Cellular Localisation | CytoplasmMitochondrion MatrixMitochondrion Intermembrane SpacePeripheral Membrane Protein |
| Alternative Protein Names | Polyribonucleotide Nucleotidyltransferase 1 - Mitochondrial protein3'-5' Rna Exonuclease Old35 proteinPnpase Old-35 proteinPolynucleotide Phosphorylase 1 proteinPnpase 1 proteinPolynucleotide Phosphorylase-Like Protein proteinPNPT1 proteinPNPASE protein |
Information sourced from Uniprot.org