Human PLA2G6 protein (Recombinant) (N-His) (STJP006546)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PLA2G6-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | PLA2G6 |
| Gene ID | 8398 |
| Uniprot ID | PLPL9_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp484-Val701 |
Additional Info
| Function | Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content. Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species. Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle. Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin. Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis. Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types. Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent pro-inflammatory signaling lipid. In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion. Isoform Ankyrin-iPLA2-1: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. Isoform Ankyrin-iPLA2-2: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. |
| Protein Name | 85/88 Kda Calcium-Independent Phospholipase A2Cai-Pla22-Lysophosphatidylcholine AcylhydrolaseGroup Vi Phospholipase A2Gvi Pla2Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 BetaIpla2-BetaPalmitoyl-Coa HydrolasePatatin-Like Phospholipase Domain-Containing Protein 9Pnpla9 |
| Database Links | Reactome: R-HSA-1482788Reactome: R-HSA-1482798Reactome: R-HSA-1482839Reactome: R-HSA-2029485Reactome: R-HSA-6811436 |
| Cellular Localisation | CytoplasmCell MembraneMitochondrionCell ProjectionPseudopodiumRecruited To The Membrane-Enriched Pseudopods Upon Mcp1/Ccl2 Stimulation In Monocytes |
| Alternative Protein Names | 85/88 Kda Calcium-Independent Phospholipase A2 proteinCai-Pla2 protein2-Lysophosphatidylcholine Acylhydrolase proteinGroup Vi Phospholipase A2 proteinGvi Pla2 proteinIntracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta proteinIpla2-Beta proteinPalmitoyl-Coa Hydrolase proteinPatatin-Like Phospholipase Domain-Containing Protein 9 proteinPnpla9 proteinPLA2G6 proteinPLPLA9 protein |
Information sourced from Uniprot.org