Human PLA2G6 protein (Recombinant) (N-His) (STJP006546)

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STJP006546
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Host: E. coli
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Recombinant-Human PLA2G6-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications.
Formulation: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage Instruction: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.
Gene Symbol: PLA2G6
Gene ID: 8398
Uniprot ID: PLPL9_HUMAN
Immunogen Region: Asp484-Val701
Immunogen: Homo sapiens (Human)
Function Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content. Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species. Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle. Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin. Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis. Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types. Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent pro-inflammatory signaling lipid. In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion. Isoform Ankyrin-iPLA2-1: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. Isoform Ankyrin-iPLA2-2: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms.
Protein Name 85/88 Kda Calcium-Independent Phospholipase A2
Cai-Pla2
2-Lysophosphatidylcholine Acylhydrolase
Group Vi Phospholipase A2
Gvi Pla2
Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta
Ipla2-Beta
Palmitoyl-Coa Hydrolase
Patatin-Like Phospholipase Domain-Containing Protein 9
Pnpla9
Database Links Reactome: R-HSA-1482788
Reactome: R-HSA-1482798
Reactome: R-HSA-1482839
Reactome: R-HSA-2029485
Reactome: R-HSA-6811436
Cellular Localisation Cytoplasm
Cell Membrane
Mitochondrion
Cell Projection
Pseudopodium
Recruited To The Membrane-Enriched Pseudopods Upon Mcp1/Ccl2 Stimulation In Monocytes
Alternative Protein Names 85/88 Kda Calcium-Independent Phospholipase A2 protein
Cai-Pla2 protein
2-Lysophosphatidylcholine Acylhydrolase protein
Group Vi Phospholipase A2 protein
Gvi Pla2 protein
Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta protein
Ipla2-Beta protein
Palmitoyl-Coa Hydrolase protein
Patatin-Like Phospholipase Domain-Containing Protein 9 protein
Pnpla9 protein
PLA2G6 protein
PLPLA9 protein

Information sourced from Uniprot.org