Human NEIL1 protein (Recombinant) (His-Tag) (STJP017470)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP017470
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General Information

Short DescriptionRecombinant-Human NEIL1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% Glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNEIL1
Gene ID79661
Uniprot IDNEIL1_HUMAN
Accession NumberNP_078884.2
ImmunogenHuman
Immunogen Region1-390aa
Immunogen Sequence MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRP

Additional Info

Tissue Specificity Ubiquitous.
Function Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as a DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.
Protein Name Endonuclease 8-Like 1
Dna Glycosylase/Ap Lyase Neil1
Dna-(Apurinic Or Apyrimidinic Site Lyase Neil1
Endonuclease Viii-Like 1
Fpg1
Nei Homolog 1
Neh1
Nei-Like Protein 1
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-5649702
Reactome: R-HSA-9616334
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
Chromosome
During Mitosis
Associates With Centrosomes And Condensed Chromatin
Alternative Protein Names Endonuclease 8-Like 1 protein
Dna Glycosylase/Ap Lyase Neil1 protein
Dna-(Apurinic Or Apyrimidinic Site Lyase Neil1 protein
Endonuclease Viii-Like 1 protein
Fpg1 protein
Nei Homolog 1 protein
Neh1 protein
Nei-Like Protein 1 protein
NEIL1 protein

Information sourced from Uniprot.org

Citations

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