Human MCP-1/CCL2 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001547)
SPECIFICATIONS
HostHEK293
ConjugationBiotin
ImmunogenDNA sequence encoding Human MCP-1/CCL2 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
General Information
| Short Description | Recombinant-Human MCP-1/CCL2-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Dilution Range | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Formulation | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Storage Instruction | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
Target Information
| Gene Symbol | CCL2 |
| Gene ID | 6347 |
| Uniprot ID | CCL2_HUMAN |
| Immunogen | DNA sequence encoding Human MCP-1/CCL2 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Immunogen Region | Gln24-Thr99 |
Additional Info
| Post Translational Modifications | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. N-Glycosylated. |
| Function | Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Protein Name | C-C Motif Chemokine 2Hc11Monocyte Chemoattractant Protein 1Monocyte Chemotactic And Activating FactorMcafMonocyte Chemotactic Protein 1Mcp-1Monocyte Secretory Protein JeSmall-Inducible Cytokine A2 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9818026 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | C-C Motif Chemokine 2 proteinHc11 proteinMonocyte Chemoattractant Protein 1 proteinMonocyte Chemotactic And Activating Factor proteinMcaf proteinMonocyte Chemotactic Protein 1 proteinMcp-1 proteinMonocyte Secretory Protein Je proteinSmall-Inducible Cytokine A2 proteinCCL2 proteinMCP1 proteinSCYA2 protein |
Information sourced from Uniprot.org