Human KIAA0101/PCLAF protein (Recombinant) (His-Tag) (STJP018407)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human KIAA0101/PCLAF-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PCLAF |
| Gene ID | 9768 |
| Uniprot ID | PAF15_HUMAN |
| Accession Number | NP_055551.1 |
| Immunogen | Human |
| Immunogen Region | 1-111aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed predominantly in liver, pancreas and placenta. Not detected in heart or brain. Highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas, adrenocortical carcinomas, and in non-small-cell lung cancer lines. |
| Post Translational Modifications | Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome. |
| Function | PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number. |
| Protein Name | Pcna-Associated FactorHepatitis C Virus Ns5a-Transactivated Protein 9Hcv Ns5a-Transactivated Protein 9Overexpressed In Anaplastic Thyroid Carcinoma 1Oeatc-1Pcna-Associated Factor Of 15 KdaPaf15P15pafPcna-Clamp-Associated Factor |
| Database Links | Reactome: R-HSA-5656169 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionFollowing Dna DamageLocalizes To Dna Damage SitesColocalizes With Centrosomes In Perinuclear Region |
| Alternative Protein Names | Pcna-Associated Factor proteinHepatitis C Virus Ns5a-Transactivated Protein 9 proteinHcv Ns5a-Transactivated Protein 9 proteinOverexpressed In Anaplastic Thyroid Carcinoma 1 proteinOeatc-1 proteinPcna-Associated Factor Of 15 Kda proteinPaf15 proteinP15paf proteinPcna-Clamp-Associated Factor proteinPCLAF proteinKIAA0101 proteinNS5ATP9 proteinPAF proteinL5 protein |
Information sourced from Uniprot.org