Human KARS1/Lysyl-tRNA synthetase protein (Recombinant) (C-His) (STJP013474)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human KARS1/Lysyl-tRsynthetase-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Supplied as a 0.22 Mu m filtered solution in 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | KARS1 |
| Gene ID | 3735 |
| Uniprot ID | SYK_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ala2-Val597 |
Additional Info
| Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity. (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. |
| Protein Name | Lysine--Trna LigaseLysyl-Trna SynthetaseLysrs |
| Database Links | Reactome: R-HSA-2408522Reactome: R-HSA-379716Reactome: R-HSA-379726Reactome: R-HSA-9856649 |
| Cellular Localisation | Isoform Cytoplasmic: CytoplasmCytosolCytoplasmNucleusCell MembranePeripheral Membrane ProteinSecretedSecretion Is Induced By Tnf-AlphaCytosolic In Quiescent Mast CellsTranslocates Into The Nucleus In Response To Mast Cell Activation By Immunoglobulin EIsoform Mitochondrial: Mitochondrion |
| Alternative Protein Names | Lysine--Trna Ligase proteinLysyl-Trna Synthetase proteinLysrs proteinKARS1 proteinKARS proteinKIAA0070 protein |
Information sourced from Uniprot.org