Human Isocitrate Dehydrogenase 1/IDH1 protein (Recombinant) (His-Tag) (STJP017408)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Isocitrate Dehydrogenase 1/IDH1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% Glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 18, 000pmol/min/ug. One unit will oxidize 1.0pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C. |
Target Information
| Gene Symbol | IDH1 |
| Gene ID | 3417 |
| Uniprot ID | IDHC_HUMAN |
| Accession Number | NP_005887.2 |
| Immunogen | Human |
| Immunogen Region | 1-414aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Acetylation at Lys-374 dramatically reduces catalytic activity. |
| Function | Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency. |
| Protein Name | Isocitrate Dehydrogenase Nadp CytoplasmicIdhIdh1Cytosolic Nadp-Isocitrate DehydrogenaseIdpcNadp(+-Specific IcdhOxalosuccinate Decarboxylase |
| Database Links | Reactome: R-HSA-2978092Reactome: R-HSA-389542Reactome: R-HSA-6798695Reactome: R-HSA-9033241Reactome: R-HSA-9818025 |
| Cellular Localisation | CytoplasmCytosolPeroxisome |
| Alternative Protein Names | Isocitrate Dehydrogenase Nadp Cytoplasmic proteinIdh proteinIdh1 proteinCytosolic Nadp-Isocitrate Dehydrogenase proteinIdpc proteinNadp(+-Specific Icdh proteinOxalosuccinate Decarboxylase proteinIDH1 proteinPICD protein |
Information sourced from Uniprot.org